Mouse Anti-ARPC5 Recombinant Antibody (2G1) (CBMAB-P0076-YC)

Name: Mouse Anti-ARPC5 Recombinant Antibody (2G1)
SKU: CBMAB-P0076-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2G1

Application

WB, IHC

Immunogen

Full length human recombinant protein of human ARPC5 (NP_005708) produced in HEK293T cell.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Actin Related Protein 2/3 Complex Subunit 5

Introduction

ARPC5 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

Entrez Gene ID

10092

UniProt ID

O15511

Alternative Names

5830443F10Rik; p16-Arc

Function

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079).
The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079).
In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947).
The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

Biological Process

Actin cytoskeleton organization Source: ProtInc
Actin filament network formation Source: Ensembl
Arp2/3 complex-mediated actin nucleation Source: FlyBase
Cell migration Source: CACAO
Ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Lamellipodium organization Source: Ensembl
Maintenance of cell polarity Source: Ensembl
Membrane organization Source: Reactome
Microtubule organizing center localization Source: Ensembl
Neutrophil degranulation Source: Reactome
Orbitofrontal cortex development Source: Ensembl
Smooth muscle cell migration Source: Ensembl

Cellular Location

Cytoskeleton; Nucleus; Cell projection

PTM

Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.

Lui, J. W., Moore, S. P., Huang, L., Ogomori, K., Li, Y., & Lang, D. (2021). YAP facilitates melanoma migration through regulation of actin‐related protein 2/3 complex subunit 5 (ARPC5). Pigment Cell & Melanoma Research.

von Loeffelholz, O., Purkiss, A., Cao, L., Kjaer, S., Kogata, N., Romet-Lemonne, G., ... & Moores, C. A. (2020). Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms. Biology open, 9(7), bio054304.

Xiong, T., & Luo, Z. (2018). The expression of actin-related protein 2/3 complex subunit 5 (ARPC5) expression in multiple myeloma and its prognostic significance. Medical science monitor: international medical journal of experimental and clinical research, 24, 6340.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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