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Mouse Anti-ARPC5 Recombinant Antibody (CBYC-P023) (CBMAB-P0078-YC)

Provided herein is a Mouse monoclonal antibody against Human Actin Related Protein 2/3 Complex Subunit 5. The antibody can be used for immunoassay techniques, such as ICC, WB.
See all ARPC5 antibodies
Published Data
Mouse Anti-ARPC5 Recombinant Antibody (CBYC-P023) CBMAB-P0078-YC in Immunoblot
EML cells were transduced with Cas9 control, TXN1 specific CRISPR/Cas9, p53 specific CRISPR/cas9 or both. p53 and P53 downstream signaling pathway were assessed by immunoblot. β-actin was used to assess equal loading. ...View More
Jabbar, S., Mathews, P., Wang, X., Sundaramoorthy, P., Chu, E., Piryani, S. O., ... & Kang, Y. (2022). Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor. Experimental Hematology & Oncology, 11(1), 1-22.
Mouse Anti-ARPC5 Recombinant Antibody (CBYC-P023) CBMAB-P0078-YC in IHC
Representative immunohistochemistry images and score distribution of ARPC5 expression in paracarcinoma normal tissues (PN) and CRC tumors (tumor). The median and quartile values in each group of individuals are shown as thick red dash lines and thin black dotted lines, respectively. Scale bar: 50 μm. The statistical analysis was performed by two-sided Mann-Whitney rank test. ...View More
Sun, Y., Guo, Z., Liu, X., Yang, L., Jing, Z., Cai, M., ... & Zhao, X. (2022). Noninvasive urinary protein signatures associated with colorectal cancer diagnosis and metastasis. Nature communications, 13(1), 2757.
Mouse Anti-ARPC5 Recombinant Antibody (CBYC-P023) CBMAB-P0078-YC in IF
Immunofluorescence using antibody against p16 and p21 was analyzed in the gingival epithelium of diabetic and control patients. Scale bar, 200 μm. ...View More
Zhang, P., Lu, B., Zhu, R., Yang, D., Liu, W., Wang, Q., ... & Wang, Q. (2021). Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation. Journal of Periodontal Research, 56(4), 667-678.
Mouse Anti-ARPC5 Recombinant Antibody (CBYC-P023) CBMAB-P0078-YC in WB
Western blotting analysis showing p16- and p21-specific immunoreactivity in the gingival epithelium of diabetic and control mice. The optical density (O.D.) values of p16 and p21 levels relative to β-actin are represented in bar histograms. The data are means ±SD (n = 3). *p < 0.05 versus control mice. ...View More
Zhang, P., Lu, B., Zhu, R., Yang, D., Liu, W., Wang, Q., ... & Wang, Q. (2021). Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation. Journal of Periodontal Research, 56(4), 667-678.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-P023
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-151 representing full length p16-ARC of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Actin Related Protein 2/3 Complex Subunit 5
Introduction
ARPC5 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene.
Entrez Gene ID
Human10092
Mouse67771
Rat360854
Hamster100766799
UniProt ID
HumanO15511
MouseQ9CPW4
RatQ4KLF8
HamsterA0A1U7QR71
Alternative Names
Actin Related Protein 2/3 Complex Subunit 5; Arp2/3 Protein Complex Subunit P16; Arp2/3 Complex 16 KDa Subunit; P16-Arc; ARC16; Actin Related Protein 2/3 Complex, Subunit 5 (16 KD);
Function
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079).
The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079).
In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947).
The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Biological Process
Actin cytoskeleton organization Source: ProtInc
Actin filament network formation Source: Ensembl
Arp2/3 complex-mediated actin nucleation Source: FlyBase
Cell migration Source: CACAO
Ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Lamellipodium organization Source: Ensembl
Maintenance of cell polarity Source: Ensembl
Membrane organization Source: Reactome
Microtubule organizing center localization Source: Ensembl
Neutrophil degranulation Source: Reactome
Orbitofrontal cortex development Source: Ensembl
Smooth muscle cell migration Source: Ensembl
Cellular Location
Cytoskeleton; Nucleus; Cell projection
PTM
Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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