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Mouse Anti-ASAP1 Recombinant Antibody (E12) (CBMAB-XB0059-YC)

Provided herein is a Mouse Recombinant Antibody against ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1. The antibody can be used for immunoassay techniques, such as ELISA, IHC, IP, WB.
See all ASAP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
E12
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 837-873 near the C-terminus of DDEF1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1
Introduction
ASAP1 is an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling.
Entrez Gene ID
Human50807
Rat314961
UniProt ID
HumanQ9ULH1
RatQ1AAU6
Alternative Names
ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1; 130 KDa Phosphatidylinositol 4,5-Bisphosphate-Dependent ARF1 GTPase-Activating Protein; ADP-Ribosylation Factor-Directed GTPase-Activating Protein 1; Development And Differentiation-Enhancing Factor 1; ARF GTPase-Activating Protein 1; PIP2-Dependent ARF1 GAP; Centaurin, Beta 4; DDEF1; DEF-1; 130 KDa Phosphatidylinositol 4,5-Biphosphate-Dependent ARF1 GTPase-Activating Protein;
Function
Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).
Plays a role in ciliogenesis.
Biological Process
Cilium assembly Source: UniProtKB
Negative regulation of dendritic spine development Source: Ensembl
Positive regulation of membrane tubulation Source: GO_Central
Positive regulation of podosome assembly Source: GO_Central
Cellular Location
Cytoplasm; Membrane. Predominantly cytoplasmic. Partially membrane-associated.
PTM
Phosphorylated on tyrosine residues by SRC.

Jiang, N., Yang, Y., Zhao, G., Yuan, Q., Liu, Z., Wang, X., ... & Fan, Y. (2020). Knockout of ASAP1 induces autophagy in papillary thyroid carcinoma by inhibiting the mTOR signaling pathway. Pathology-Research and Practice, 216(6), 152950.

Soubias, O., Pant, S., Heinrich, F., Zhang, Y., Roy, N. S., Li, J., ... & Byrd, R. A. (2020). Membrane surface recognition by the ASAP1 PH domain and consequences for interactions with the small GTPase Arf1. Science advances, 6(40), eabd1882.

Chen, P. W., Billington, N., Maron, B. Y., Sload, J. A., Chinthalapudi, K., & Heissler, S. M. (2020). The BAR domain of the Arf GTPase-activating protein ASAP1 directly binds actin filaments. Journal of Biological Chemistry, 295(32), 11303-11315.

Cui, J., Wen, D., Wang, Y., & Wu, C. (2019). ASAP1 knockdown reduces migration of RAW264. 7 cells infected with Mycobacterium tuberculosis. Xi bao yu fen zi mian yi xue za zhi= Chinese journal of cellular and molecular immunology, 35(12), 1064-1068.

Gao, H., Qin, L., Shi, H., Zhang, H., Li, C., Ma, Y., ... & Liu, T. (2019). ASAP1 promotes tumor progression and angiogenesis and independently predicts poor prognosis and lymph node metastasis of gastric cancer.

Roy, N. S., Jian, X., Soubias, O., Zhai, P., Hall, J. R., Dagher, J. N., ... & Randazzo, P. A. (2019). Interaction of the N terminus of ADP-ribosylation factor with the PH domain of the GTPase-activating protein ASAP1 requires phosphatidylinositol 4, 5-bisphosphate. Journal of Biological Chemistry, 294(46), 17354-17370.

Gasilina, A., Vitali, T., Luo, R., Jian, X., & Randazzo, P. A. (2019). The ArfGAP ASAP1 controls actin stress fiber organization via its N-BAR domain. Iscience, 22, 166-180.

Zhang, T., Zhao, G., Yang, C., Dong, P., Watari, H., Zeng, L., ... & Yue, J. (2018). Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells. Oncology letters, 15(4), 4432-4438.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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