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Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) (CBMAB-A3710-YC)

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Published Data
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Protein complex was pulled down with ASAP1 antibody and then blotted with both FAK and ASAP1 antibody. (*p<0.05; **p<0.01; ***p<0.001). ...View More
Huo, X., Zhang, W., Zhao, G., Chen, Z., Dong, P., Watari, H., ... & Yue, J. (2022). FAK PROTAC inhibits ovarian tumor growth and metastasis by disrupting kinase dependent and independent pathways. Frontiers in oncology, 12, 851065.
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Western blot analysis at 3 h postinfection using specific antibodies. ...View More
Cui, J., Chen, G., Zhao, Z., Wang, L., Wen, D., Xing, L., & Wu, C. (2021). ASAP1 regulates the uptake of Mycobacterium tuberculosis H37Ra in THP1-derived macrophages by remodeling actin cytoskeleton. Tuberculosis, 129, 102090.
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Mouse Anti-ASAP1 Recombinant Antibody (CBYC-A806) CBMAB-A3710-YC in WB
Western blotting analysis of the knockdown efficiency of asap1 morphants in 1, 5, and 10 dpf zebrafish. *P < 0.05, **P < 0.01 vs. control morphants using Student's unpaired t-test. ...View More
Cui, J., Chen, G., Wen, D., Wang, Y., Zhao, Z., & Wu, C. (2020). Asap1 Affects the Susceptibility of Zebrafish to Mycobacterium by Regulating Macrophage Migration. Frontiers in Cellular and Infection Microbiology, 10, 519503.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBYC-A806
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 837-873 near the C-terminus of DDEF1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1030-1130
More Infomation

Target

Full Name
ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1
Introduction
ASAP1 is an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regula
Entrez Gene ID
50807
UniProt ID
Q9ULH1
Alternative Names
ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1; 130 KDa Phosphatidylinositol 4,5-Bisphosphate-Dependent ARF1 GTPase-Activating Protein; ADP-Ribosylation Factor-Directed GTPase-Activating Protein 1; Development And Differentiation-Enhancing Factor
Function
Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).
Plays a role in ciliogenesis.
Biological Process
Cilium assembly Source: UniProtKB
Negative regulation of dendritic spine development Source: Ensembl
Positive regulation of membrane tubulation Source: GO_Central
Positive regulation of podosome assembly Source: GO_Central
Cellular Location
Cytoplasm; Membrane. Predominantly cytoplasmic. Partially membrane-associated.
PTM
Phosphorylated on tyrosine residues by SRC.

Jiang, N., Yang, Y., Zhao, G., Yuan, Q., Liu, Z., Wang, X., ... & Fan, Y. (2020). Knockout of ASAP1 induces autophagy in papillary thyroid carcinoma by inhibiting the mTOR signaling pathway. Pathology-Research and Practice, 216(6), 152950.

Soubias, O., Pant, S., Heinrich, F., Zhang, Y., Roy, N. S., Li, J., ... & Byrd, R. A. (2020). Membrane surface recognition by the ASAP1 PH domain and consequences for interactions with the small GTPase Arf1. Science advances, 6(40), eabd1882.

Chen, P. W., Billington, N., Maron, B. Y., Sload, J. A., Chinthalapudi, K., & Heissler, S. M. (2020). The BAR domain of the Arf GTPase-activating protein ASAP1 directly binds actin filaments. Journal of Biological Chemistry, 295(32), 11303-11315.

Cui, J., Wen, D., Wang, Y., & Wu, C. (2019). ASAP1 knockdown reduces migration of RAW264. 7 cells infected with Mycobacterium tuberculosis. Xi bao yu fen zi mian yi xue za zhi= Chinese journal of cellular and molecular immunology, 35(12), 1064-1068.

Gao, H., Qin, L., Shi, H., Zhang, H., Li, C., Ma, Y., ... & Liu, T. (2019). ASAP1 promotes tumor progression and angiogenesis and independently predicts poor prognosis and lymph node metastasis of gastric cancer.

Roy, N. S., Jian, X., Soubias, O., Zhai, P., Hall, J. R., Dagher, J. N., ... & Randazzo, P. A. (2019). Interaction of the N terminus of ADP-ribosylation factor with the PH domain of the GTPase-activating protein ASAP1 requires phosphatidylinositol 4, 5-bisphosphate. Journal of Biological Chemistry, 294(46), 17354-17370.

Gasilina, A., Vitali, T., Luo, R., Jian, X., & Randazzo, P. A. (2019). The ArfGAP ASAP1 controls actin stress fiber organization via its N-BAR domain. Iscience, 22, 166-180.

Zhang, T., Zhao, G., Yang, C., Dong, P., Watari, H., Zeng, L., ... & Yue, J. (2018). Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells. Oncology letters, 15(4), 4432-4438.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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