Mouse Anti-ASMT Recombinant Antibody (1F8) (CBMAB-A3787-YC)

Name: Mouse Anti-ASMT Recombinant Antibody (1F8)
SKU: CBMAB-A3787-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F8

Application

ELISA

Immunogen

Partial recombinant corresponding to aa 71-170 from human ASMT with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

acetylserotonin O-methyltransferase

Introduction

ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant i

Entrez Gene ID

438

UniProt ID

P46597

Alternative Names

Acetylserotonin O-Methyltransferase; Hydroxyindole O-Methyltransferase; HIOMT; Acetylserotonin Methyltransferase (Y Chromosome); Acetylserotonin N-Methyltransferase; EC 2.1.1.4; HIOMTY; ASMTY;

Function

Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity.

Biological Process

Aromatic compound biosynthetic process Source: GO_Central
Indolalkylamine biosynthetic process Source: Reactome
Melatonin biosynthetic process Source: UniProtKB
Methylation Source: GO_Central
Translation Source: ProtInc

Cellular Location

Cytosol

Zhou, W., Wang, Y., Li, B., Petijová, L., Hu, S., Zhang, Q., ... & Wang, Z. (2021). Whole‐genome sequence data of Hypericum perforatum and functional characterization of melatonin biosynthesis by N‐acetylserotonin O‐methyltransferase. Journal of Pineal Research, 70(2), e12709.

Coelho, L. A., Andrade-Silva, J., Motta-Teixeira, L. C., Amaral, F. G., Reiter, R. J., & Cipolla-Neto, J. (2019). The absence of pineal melatonin abolishes the daily rhythm of tph1 (tryptophan hydroxylase 1), ASMT (acetylserotonin o-methyltransferase), and AANAT (aralkylamine n-acetyltransferase) mRNA expressions in rat testes. Molecular neurobiology, 56(11), 7800-7809.

Chen, H. L., Yuan, C. Y., Cheng, H. H., Chang, T. C., Huang, S. K., Kuo, C. C., & Wu, K. K. (2018). Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression. Journal of Biological Chemistry, 293(28), 11131-11142.

Kurtulus Dereli, A., Demırci, G. N., Dodurga, Y., Özbal, S., Cankurt, U., Boz, B., ... & Acar, K. (2018). Evaluation of human pineal gland acetylserotonin O-methyltransferase immunoreactivity in suicide: A preliminary study. Medicine, Science and the Law, 58(4), 233-238.

Lee, K., Choi, G. H., & Back, K. (2017). Cadmium‐induced melatonin synthesis in rice requires light, hydrogen peroxide, and nitric oxide: key regulatory roles for tryptophan decarboxylase and caffeic acid O‐methyltransferase. Journal of pineal research, 63(4), e12441.

Zhang, K., Ruan, Z., Li, J., Bian, C., You, X., Coon, S. L., & Shi, Q. (2017). A comparative genomic and transcriptomic survey provides novel insights into N-Acetylserotonin methyltransferase (ASMT) in fish. Molecules, 22(10), 1653.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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