Human ASMT ELISA Kit (V2LY-0626-LY5583)

Name: Human ASMT ELISA Kit
SKU: V2LY-0626-LY5583
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.016 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

38.5 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

acetylserotonin O-methyltransferase

Function

Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity.

Biological Process

Aromatic compound biosynthetic process Source: GO_Central
Indolalkylamine biosynthetic process Source: Reactome
Melatonin biosynthetic process Source: UniProtKB
Methylation Source: GO_Central
Translation Source: ProtInc

Cellular Location

Cytosol

Zhou, W., Wang, Y., Li, B., Petijová, L., Hu, S., Zhang, Q., ... & Wang, Z. (2021). Whole‐genome sequence data of Hypericum perforatum and functional characterization of melatonin biosynthesis by N‐acetylserotonin O‐methyltransferase. Journal of Pineal Research, 70(2), e12709.

Coelho, L. A., Andrade-Silva, J., Motta-Teixeira, L. C., Amaral, F. G., Reiter, R. J., & Cipolla-Neto, J. (2019). The absence of pineal melatonin abolishes the daily rhythm of tph1 (tryptophan hydroxylase 1), ASMT (acetylserotonin o-methyltransferase), and AANAT (aralkylamine n-acetyltransferase) mRNA expressions in rat testes. Molecular neurobiology, 56(11), 7800-7809.

Chen, H. L., Yuan, C. Y., Cheng, H. H., Chang, T. C., Huang, S. K., Kuo, C. C., & Wu, K. K. (2018). Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression. Journal of Biological Chemistry, 293(28), 11131-11142.

Kurtulus Dereli, A., Demırci, G. N., Dodurga, Y., Özbal, S., Cankurt, U., Boz, B., ... & Acar, K. (2018). Evaluation of human pineal gland acetylserotonin O-methyltransferase immunoreactivity in suicide: A preliminary study. Medicine, Science and the Law, 58(4), 233-238.

Lee, K., Choi, G. H., & Back, K. (2017). Cadmium‐induced melatonin synthesis in rice requires light, hydrogen peroxide, and nitric oxide: key regulatory roles for tryptophan decarboxylase and caffeic acid O‐methyltransferase. Journal of pineal research, 63(4), e12441.

Zhang, K., Ruan, Z., Li, J., Bian, C., You, X., Coon, S. L., & Shi, Q. (2017). A comparative genomic and transcriptomic survey provides novel insights into N-Acetylserotonin methyltransferase (ASMT) in fish. Molecules, 22(10), 1653.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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