Mouse Anti-ASNS Recombinant Antibody (1A10) (CBMAB-A3794-YC)

Name: Mouse Anti-ASNS Recombinant Antibody (1A10)
SKU: CBMAB-A3794-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A10

Application

IF, WB

Immunogen

Full length human recombinant protein of human ASNS(NP_597680) produced in HEK293T cell.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

asparagine synthetase

Introduction

ASNS is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature.

Entrez Gene ID

440

UniProt ID

P08243

Alternative Names

Asparagine Synthetase (Glutamine-Hydrolyzing); Glutamine-Dependent Asparagine Synthetase; EC 6.3.5.4; TS11; Asparagine Synthetase [Glutamine-Hydrolyzing]; TS11 Cell Cycle Control Protein; Cell Cycle Control Protein TS11; Asparagine Synthetase; ASNSD;

Biological Process

Asparagine biosynthetic process Source: MGI
Aspartate family amino acid metabolic process Source: Reactome
Cellular response to glucose starvation Source: UniProtKB
Cellular response to hormone stimulus Source: Ensembl
Glutamine metabolic process Source: UniProtKB-KW
L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Liver development Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of mitotic cell cycle Source: UniProtKB
Response to amino acid Source: Ensembl
Response to follicle-stimulating hormone Source: Ensembl
Response to light stimulus Source: Ensembl
Response to mechanical stimulus Source: Ensembl
Response to methotrexate Source: Ensembl
Response to toxic substance Source: Ensembl

Cellular Location

Cytosol

Involvement in disease

Asparagine synthetase deficiency (ASNSD): An inborn error of asparagine biosynthesis that results in a severe neurologic disorder characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, cortical atrophy, and seizure or hyperekplexic activity.

Hope, H. C., Brownlie, R. J., Fife, C. M., Steele, L., Lorger, M., & Salmond, R. J. (2021). Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation. JCI insight, 6(9).

Hope, H. C., Brownlie, R. J., Steele, L., & Salmond, R. J. (2020). Coordination of asparagine uptake and asparagine synthetase expression is required for T cell activation. bioRxiv.

Zhu, W., Radadiya, A., Bisson, C., Nordin, B. E., Baumann, P., Imasaki, T., ... & Richards, N. G. (2018). Molecular basis of human asparagine synthetase inhibitor specificity. bioRxiv, 428508.

Alfadhel, M., & El-Hattab, A. W. (2018). Asparagine synthetase deficiency.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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