Mouse Anti-ATG13 Recombinant Antibody (A3901) (CBMAB-A3901-YC)

Provided herein is a Mouse monoclonal antibody against Human Autophagy Related 13. The antibody can be used for immunoassay techniques, such as WB, FC, ELISA, IHC.
See all ATG13 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

A3901

Antibody Isotype

IgG1

Application

WB, FC, ELISA, IHC

Basic Information

Immunogen

Purified recombinant fragment of human ATG13 (aa 339-550) expressed in E. Coli.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:100-1:500
ELISA	1:10,000
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Autophagy Related 13

Introduction

ATG13 is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy.

Entrez Gene ID

9776

UniProt ID

O75143

Alternative Names

Autophagy Related 13; KIAA0652; ATG13 Autophagy Related 13 Homolog (S. Cerevisiae); ATG13 Autophagy Related 13 Homolog; Autophagy-Related Protein 13; PARATARG8;

Function

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Biological Process

Autophagosome assembly Source: UniProtKB
Macroautophagy Source: Reactome
Mitophagy Source: GO_Central
Piecemeal microautophagy of the nucleus Source: GO_Central
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein localization to phagophore assembly site Source: GO_Central
Regulation of macroautophagy Source: Reactome
Response to mitochondrial depolarisation Source: Ensembl

Cellular Location

Cytosol; Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.

PTM

Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria.

References

Dalle Pezze, P., Karanasios, E., Kandia, V., Manifava, M., Walker, S. A., Gambardella Le Novère, N., & Ktistakis, N. T. (2021). ATG13 dynamics in nonselective autophagy and mitophagy: insights from live imaging studies and mathematical modeling. Autophagy, 17(5), 1131-1141.

Li, Z., Tian, X., Ji, X., Wang, J., Chen, H., Wang, D., & Zhang, X. (2020). ULK1-ATG13 and their mitotic phospho-regulation by CDK1 connect autophagy to cell cycle. PLoS biology, 18(6), e3000288.

Woo, M., Choi, H. I., Park, S. H., Ahn, J., Jang, Y. J., Ha, T. Y., ... & Jung, C. H. (2020). The unc-51 like autophagy activating kinase 1-autophagy related 13 complex has distinct functions in tunicamycin-treated cells. Biochemical and Biophysical Research Communications, 524(3), 744-749.

Su, Y., Lu, J., Gong, P., Chen, X., Liang, C., & Zhang, J. (2018). Rapamycin induces autophagy to alleviate acute kidney injury following cerebral ischemia and reperfusion via the mTORC1/ATG13/ULK1 signaling pathway. Molecular medicine reports, 18(6), 5445-5454.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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