Mouse Atg13 ELISA Kit (V2LY-0626-LY3319)

Name: Mouse Atg13 ELISA Kit
SKU: V2LY-0626-LY3319
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0028 ng/mL

Detection Range

0.005-1 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

56.4 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Autophagy Related 13

Function

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Biological Process

Autophagosome assembly Source: UniProtKB
Macroautophagy Source: Reactome
Mitophagy Source: GO_Central
Piecemeal microautophagy of the nucleus Source: GO_Central
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein localization to phagophore assembly site Source: GO_Central
Regulation of macroautophagy Source: Reactome
Response to mitochondrial depolarisation Source: Ensembl

Cellular Location

Cytosol; Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.

PTM

Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria.

Dalle Pezze, P., Karanasios, E., Kandia, V., Manifava, M., Walker, S. A., Gambardella Le Novère, N., & Ktistakis, N. T. (2021). ATG13 dynamics in nonselective autophagy and mitophagy: insights from live imaging studies and mathematical modeling. Autophagy, 17(5), 1131-1141.

Li, Z., Tian, X., Ji, X., Wang, J., Chen, H., Wang, D., & Zhang, X. (2020). ULK1-ATG13 and their mitotic phospho-regulation by CDK1 connect autophagy to cell cycle. PLoS biology, 18(6), e3000288.

Woo, M., Choi, H. I., Park, S. H., Ahn, J., Jang, Y. J., Ha, T. Y., ... & Jung, C. H. (2020). The unc-51 like autophagy activating kinase 1-autophagy related 13 complex has distinct functions in tunicamycin-treated cells. Biochemical and Biophysical Research Communications, 524(3), 744-749.

Su, Y., Lu, J., Gong, P., Chen, X., Liang, C., & Zhang, J. (2018). Rapamycin induces autophagy to alleviate acute kidney injury following cerebral ischemia and reperfusion via the mTORC1/ATG13/ULK1 signaling pathway. Molecular medicine reports, 18(6), 5445-5454.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ATG13 protein, GST Tag (CAT#: V2LY-0526-LY2193)

Human Recombinant ATG13 protein, GST Tag-1 (CAT#: V2LY-0526-LY2194)

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Human Recombinant ATG13 protein, GST Tag-1 (CAT#: V2LY-0526-LY2194)

Mouse Anti-ATG13 Recombinant Antibody (CBT3137) (CAT#: V2LY-0625-LY380)

Mouse Anti-ATG13 Recombinant Antibody (A3901) (CAT#: CBMAB-A3901-YC)

Human Recombinant ATG13 protein, GST Tag (CAT#: V2LY-0526-LY2193)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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