Mouse Anti-ATIC Recombinant Antibody (1D2) (CBMAB-A3958-YC)

Name: Mouse Anti-ATIC Recombinant Antibody (1D2)
SKU: CBMAB-A3958-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D2

Application

FC, WB

Immunogen

Full length human recombinant protein of human ATIC(NP_004035) produced in HEK293T cell.

Specificity

Human, Mouse, Rat, Dog, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase

Introduction

ATIC is a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase act

Entrez Gene ID

471

UniProt ID

P31939

Alternative Names

5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase; Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase; PURH; 5-Aminoimidazole-4-Carboxamide-1-Beta-D-Ribonucleotide Transformylase/Inosinicase; AICA

Function

Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis (PubMed:11948179, PubMed:14756554).
Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR) (PubMed:9378707, PubMed:11948179, PubMed:10985775).
Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction (PubMed:10985775).
Also catalyzes the cyclization of FAICAR to IMP (PubMed:11948179, PubMed:14756554).
Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias (PubMed:10985775).
Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed:25687571).

Biological Process

de novo' IMP biosynthetic process Source: GO_Central
Animal organ regeneration Source: Ensembl
Brainstem development Source: Ensembl
Cellular response to interleukin-7 Source: Ensembl
Cerebellum development Source: Ensembl
Cerebral cortex development Source: Ensembl
Dihydrofolate metabolic process Source: Ensembl
Nucleobase-containing compound metabolic process Source: ProtInc
Nucleoside metabolic process Source: Ensembl
Purine ribonucleoside monophosphate biosynthetic process Source: Reactome
Response to inorganic substance Source: Ensembl
Tetrahydrofolate biosynthetic process Source: Ensembl

Cellular Location

Cytosol; Extracellular exosome; Plasma membrane; Membrane

Involvement in disease

AICAR transformylase/IMP cyclohydrolase deficiency (AICAR): A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.

Li, R., Chen, G., Dang, Y., He, R., Liu, A., Ma, J., & Wang, C. (2021). Upregulation of ATIC in multiple myeloma tissues based on tissue microarray and gene microarrays. International Journal of Laboratory Hematology, 43(3), 409-417.

Wu, X., Zhou, H., He, Z., Zhang, Z., Feng, W., Zhao, J., ... & Wang, Q. (2020). Coexistence of a novel CCNY-ALK and ATIC-ALK double-fusion in one patient with ALK-positive NSCLC and response to crizotinib: a case report. Translational Lung Cancer Research, 9(6), 2494.

Witkowska, D., Cox, H. L., Hall, T. C., Wildsmith, G. C., Machin, D. C., & Webb, M. E. (2018). Analysis of substrate binding in individual active sites of bifunctional human ATIC. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1866(2), 254-263.

Liu, X., Paila, U. D., Teraoka, S. N., Wright, J. A., Huang, X., Quinlan, A. R., ... & Concannon, P. (2018). Identification of ATIC as a Novel Target for Chemoradiosensitization. International Journal of Radiation Oncology* Biology* Physics, 100(1), 162-173.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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