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Mouse Anti-ATIC Recombinant Antibody (CBLQA-001) (CBMAB-2044CQ)

This product is a mouse monoclonal antibody that recognizes ATIC. The antibody CBLQA-001 can be used for immunoassay techniques such as: IHC, WB.
See all ATIC antibodies
Published Data
Mouse Anti-ATIC Recombinant Antibody (CBLQA-001) (CBMAB-2044CQ) in WB
Western blotting and relative quantification for expression levels of γ-H2AX (S139), H2AX, p-p53 (S33), p53, and p21Cip1 in H1975 and A549 cells treated with AICAR. ...View More
Aftab, F., Rodriguez-Fuguet, A., Silva, L., Kobayashi, I. S., Sun, J., Politi, K., ... & Zhang, W. C. (2023). An intrinsic purine metabolite AICAR blocks lung tumour growth by targeting oncoprotein mucin 1. British Journal of Cancer, 128(9), 1647-1664.
Mouse Anti-ATIC Recombinant Antibody (CBLQA-001) (CBMAB-2044CQ) in IF
Duolink ligation assay and confocal imaging for physical MUC1-JAK1 interactions. H441 cells were treated with vehicle or 1 mM AICAR for 1 h. After treatment, the cells were incubated with mouse anti-ZO-1 primary antibody overnight, followed by anti-mouse IgG conjugated with Alexa Fluor 488. Then the cells were co-incubated with anti-MUC1 and anti-JAK1 primary antibodies, followed by incubation with proximity ligation assay probes conjugated with Cy3, ligation, and amplification steps. The nucleus was counterstained with DAPI. The images were taken using a Zeiss confocal fluorescent microscope, and the Duolink dots were quantified using Image J. Scale Bar, 20 µm. ...View More
Aftab, F., Rodriguez-Fuguet, A., Silva, L., Kobayashi, I. S., Sun, J., Politi, K., ... & Zhang, W. C. (2023). An intrinsic purine metabolite AICAR blocks lung tumour growth by targeting oncoprotein mucin 1. British Journal of Cancer, 128(9), 1647-1664.
Mouse Anti-ATIC Recombinant Antibody (CBLQA-001) (CBMAB-2044CQ) in WB
Western blot analysis and quantification of PPAT, GART, PFAS, PAICS, ATIC, AMPD2, and ADSS in H1975 OTR cells treated with the LNA miR-21-5p inhibitor (anti-miR-21-5p) or a control plasmid (anti-miR-control). ...View More
Zhang, W. C., Skiados, N., Aftab, F., Moreno, C., Silva, L., Corbilla, P. J. A., ... & Slack, F. J. (2022). MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state. Cancer Gene Therapy, 29(12), 1878-1894.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBLQA-001
Antibody Isotype
IgG1, κ
Application
IP, WB, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-270 mapping at the N-terminus of AICAR transformylase of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase
Introduction
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. A mutation in this gene results in AICA-ribosiduria.Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
Entrez Gene ID
Human471
Mouse108147
Rat81643
Frog448061
UniProt ID
HumanP31939
MouseQ9CWJ9
RatO35567
FrogF6R882
Alternative Names
PURH; AICAR; AICARFT; IMPCHASE; HEL-S-70p
Function
Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis (PubMed:11948179, PubMed:14756554).
Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR) (PubMed:9378707, PubMed:11948179, PubMed:10985775).
Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction (PubMed:10985775).
Also catalyzes the cyclization of FAICAR to IMP (PubMed:11948179, PubMed:14756554).
Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias (PubMed:10985775).
Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed:25687571).
Biological Process
de novo' IMP biosynthetic process Source: GO_Central
Animal organ regeneration Source: Ensembl
Brainstem development Source: Ensembl
Cellular response to interleukin-7 Source: Ensembl
Cerebellum development Source: Ensembl
Cerebral cortex development Source: Ensembl
Dihydrofolate metabolic process Source: Ensembl
Nucleobase-containing compound metabolic process Source: ProtInc
Nucleoside metabolic process Source: Ensembl
Purine ribonucleoside monophosphate biosynthetic process Source: Reactome
Response to inorganic substance Source: Ensembl
Tetrahydrofolate biosynthetic process Source: Ensembl
Cellular Location
Cytosol; Extracellular exosome; Plasma membrane; Membrane
Involvement in disease
AICAR transformylase/IMP cyclohydrolase deficiency (AICAR): A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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