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Mouse Anti-ATIC Recombinant Antibody (F38P7H9) (CBMAB-A3962-YC)

Provided herein is a Mouse monoclonal antibody against Human 5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase. The antibody can be used for immunoassay techniques, such as FC, ICC, IF, IHC-P, WB.
See all ATIC antibodies
Published Data
Mouse Anti-ATIC Recombinant Antibody (F38P7H9) (CBMAB-A3962-YC) in WB
Western blot and densitometry analyses of CPS2 and HPRT protein levels in the hippocampus, prefrontal cortex and erythrocytes. ...View More
Park, D. I., Dournes, C., Sillaber, I., Uhr, M., Asara, J. M., Gassen, N. C., ... & Turck, C. W. (2016). Purine and pyrimidine metabolism: Convergent evidence on chronic antidepressant treatment response in mice and humans. Scientific reports, 6(1), 35317.
Mouse Anti-ATIC Recombinant Antibody (F38P7H9) (CBMAB-A3962-YC) in IHC
The pictures show CLK3, p-USP13-Y708, c-Myc, and ATIC protein expression in two human CCA specimens. Bars, 100 µm. ...View More
Zhou, Q., Lin, M., Feng, X., Ma, F., Zhu, Y., Liu, X., ... & Zhang, Z. (2020). Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism. Journal of Experimental Medicine, 217(8), e20191779.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Fruit fly, Rat, Frog
Clone
F38P7H9
Antibody Isotype
IgG1, κ
Application
FC, IHC-P, IP, WB

Basic Information

Immunogen
Synthetic peptide, conjugated to Ovalbumin, corresponding to residues A(583) H T N L R L F H H(592) of human ATIC.
Specificity
Human, Mouse, Fruit fly, Rat, Frog
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB2 µg/ml
FC1 µg/10^6 cells
IHC-P5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase
Introduction
ATIC is a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase act
Entrez Gene ID
Human471
Mouse108147
Rat81643
Frog448061
UniProt ID
HumanP31939
MouseQ9CWJ9
RatO35567
FrogF6R882
Alternative Names
5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase; Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase; PURH; 5-Aminoimidazole-4-Carboxamide-1-Beta-D-Ribonucleotide Transformylase/Inosinicase; AICA
Function
Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis (PubMed:11948179, PubMed:14756554).
Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR) (PubMed:9378707, PubMed:11948179, PubMed:10985775).
Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction (PubMed:10985775).
Also catalyzes the cyclization of FAICAR to IMP (PubMed:11948179, PubMed:14756554).
Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias (PubMed:10985775).
Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed:25687571).
Biological Process
de novo' IMP biosynthetic process Source: GO_Central
Animal organ regeneration Source: Ensembl
Brainstem development Source: Ensembl
Cellular response to interleukin-7 Source: Ensembl
Cerebellum development Source: Ensembl
Cerebral cortex development Source: Ensembl
Dihydrofolate metabolic process Source: Ensembl
Nucleobase-containing compound metabolic process Source: ProtInc
Nucleoside metabolic process Source: Ensembl
Purine ribonucleoside monophosphate biosynthetic process Source: Reactome
Response to inorganic substance Source: Ensembl
Tetrahydrofolate biosynthetic process Source: Ensembl
Cellular Location
Cytosol; Extracellular exosome; Plasma membrane; Membrane
Involvement in disease
AICAR transformylase/IMP cyclohydrolase deficiency (AICAR): A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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