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Mouse Anti-ATOX1 Antibody (2E6) (CBMAB-0011-YC)

Provided herein are mouse monoclonal antibodies against Human ATOX1. The antibody clone 2E6 can be used for immunoassay techniques, such as WB.
See all ATOX1 antibodies
Published Data
Fig.1 Schematic representation of the Atox1 fusion protein (A) and expression and purification of Atox1 using the pTYB12-Atox1 construct (B).
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Fig.2 Atox1 accumulates in the lamellipodium border of MDA-MB-231 cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2E6
Antibody Isotype
IgG2a
Application
WB, ELISA, IF

Basic Information

Immunogen
ATOX1 (NP_004036, 1 a.a. ~ 68 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Antioxidant 1 Copper Chaperone
Introduction
ATOX1 is a copper metallochaperone protein that is encoded by the ATOX1 gene in humans. Homologous proteins are found in a wide variety of eukaryotes, including Saccharomyces cerevisiae as ATX1, and all contain a conserved metal binding domain. In mammals, ATOX1 plays a key role in copper homeostasis as it delivers copper from the cytosol to transporters ATP7A and ATP7B.
Entrez Gene ID
475
UniProt ID
O00244
Alternative Names
Antioxidant 1 Copper Chaperone; Metal Transport Protein ATX1; HAH1; ATX1 (Antioxidant Protein 1, Yeast) Homolog; ATX1 Antioxidant Protein 1 Homolog (Yeast); ATX1 Antioxidant Protein 1 Homolog; ATX1
Function
Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.
Biological Process
Cellular copper ion homeostasis Source: GO_Central
Copper ion transport Source: UniProtKB
Response to oxidative stress Source: GO_Central
Cellular Location
Cytosol

Blockhuys, S., Zhang, X., & Wittung-Stafshede, P. (2020). Single-cell tracking demonstrates copper chaperone Atox1 to be required for breast cancer cell migration. Proceedings of the National Academy of Sciences, 117(4), 2014-2019.

Horvath, I., Blockhuys, S., Šulskis, D., Holgersson, S., Kumar, R., Burmann, B. M., & Wittung-Stafshede, P. (2019). Interaction between copper chaperone Atox1 and Parkinson’s disease protein α-Synuclein includes metal-binding sites and occurs in living cells. ACS chemical neuroscience, 10(11), 4659-4668.

Nardella, M. I., Rosato, A., Belviso, B. D., Caliandro, R., Natile, G., & Arnesano, F. (2019). Oxidation of Human Copper Chaperone Atox1 and Disulfide Bond Cleavage by Cisplatin and Glutathione. International journal of molecular sciences, 20(18), 4390.

Das, A., Sudhahar, V., Ushio-Fukai, M., & Fukai, T. (2019). Novel interaction of antioxidant-1 with TRAF4: role in inflammatory responses in endothelial cells. American Journal of Physiology-Cell Physiology, 317(6), C1161-C1171.

Dzebo, M. M., Blockhuys, S., Valenzuela, S., Celauro, E., Esbjörner, E. K., & Wittung-Stafshede, P. (2018). Copper chaperone Atox1 interacts with cell cycle proteins. Computational and structural biotechnology journal, 16, 443-449.

Kamiya, T., Takeuchi, K., Fukudome, S., Hara, H., & Adachi, T. (2018). Copper chaperone antioxidant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells. Biometals, 31(1), 61-68.

Hatori, Y., Inouye, S., & Akagi, R. (2017). Thiol‐based copper handling by the copper chaperone Atox1. IUBMB life, 69(4), 246-254.

Blockhuys, S., & Wittung-Stafshede, P. (2017). Copper chaperone Atox1 plays role in breast cancer cell migration. Biochemical and biophysical research communications, 483(1), 301-304.

Celauro, E., Mukaj, A., Fierro-González, J. C., & Wittung-Stafshede, P. (2017). Copper chaperone ATOX1 regulates pluripotency factor OCT4 in preimplantation mouse embryos. Biochemical and biophysical research communications, 491(1), 147-153.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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