Human Recombinant ATOX1 protein, His Tag (V2LY-0526-LY2198)

Name: Human Recombinant ATOX1 protein, His Tag
SKU: V2LY-0526-LY2198
Rating: 5 (1 reviews)

See all ATOX1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ATOX1 protein, His Tag consist of Amino Acid: 1-68 and predicts a molecular mass of 9.6 kDa.

Molecule Mass

9.6 kDa

Sequence

Amino Acid: 1-68

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Antioxidant 1 Copper Chaperone

Function

Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.

Biological Process

Cellular copper ion homeostasis Source: GO_Central
Copper ion transport Source: UniProtKB
Response to oxidative stress Source: GO_Central

Cellular Location

Cytosol

Blockhuys, S., Zhang, X., & Wittung-Stafshede, P. (2020). Single-cell tracking demonstrates copper chaperone Atox1 to be required for breast cancer cell migration. Proceedings of the National Academy of Sciences, 117(4), 2014-2019.

Horvath, I., Blockhuys, S., Šulskis, D., Holgersson, S., Kumar, R., Burmann, B. M., & Wittung-Stafshede, P. (2019). Interaction between copper chaperone Atox1 and Parkinson’s disease protein α-Synuclein includes metal-binding sites and occurs in living cells. ACS chemical neuroscience, 10(11), 4659-4668.

Nardella, M. I., Rosato, A., Belviso, B. D., Caliandro, R., Natile, G., & Arnesano, F. (2019). Oxidation of Human Copper Chaperone Atox1 and Disulfide Bond Cleavage by Cisplatin and Glutathione. International journal of molecular sciences, 20(18), 4390.

Das, A., Sudhahar, V., Ushio-Fukai, M., & Fukai, T. (2019). Novel interaction of antioxidant-1 with TRAF4: role in inflammatory responses in endothelial cells. American Journal of Physiology-Cell Physiology, 317(6), C1161-C1171.

Dzebo, M. M., Blockhuys, S., Valenzuela, S., Celauro, E., Esbjörner, E. K., & Wittung-Stafshede, P. (2018). Copper chaperone Atox1 interacts with cell cycle proteins. Computational and structural biotechnology journal, 16, 443-449.

Kamiya, T., Takeuchi, K., Fukudome, S., Hara, H., & Adachi, T. (2018). Copper chaperone antioxidant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells. Biometals, 31(1), 61-68.

Hatori, Y., Inouye, S., & Akagi, R. (2017). Thiol‐based copper handling by the copper chaperone Atox1. IUBMB life, 69(4), 246-254.

Blockhuys, S., & Wittung-Stafshede, P. (2017). Copper chaperone Atox1 plays role in breast cancer cell migration. Biochemical and biophysical research communications, 483(1), 301-304.

Celauro, E., Mukaj, A., Fierro-González, J. C., & Wittung-Stafshede, P. (2017). Copper chaperone ATOX1 regulates pluripotency factor OCT4 in preimplantation mouse embryos. Biochemical and biophysical research communications, 491(1), 147-153.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant ATOX1 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ATOX1 Antibody (3E1) (CAT#: CBMAB-0016-YC)

Mouse Anti-ATOX1 Antibody (3B7) (CAT#: CBMAB-0014-YC)

Mouse Anti-ATOX1 Recombinant Antibody (S65-37) (CAT#: CBMAB-AP502LY)

Mouse Anti-ATOX1 Recombinant Antibody (3A13) (CAT#: CBMAB-A0602-LY)

Mouse Anti-ATOX1 Antibody (2E6) (CAT#: CBMAB-0011-YC)

Mouse Anti-ATOX1 Recombinant Antibody (S76-8) (CAT#: CBMAB-AP501LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry