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Mouse Anti-ATP2B1 Recombinant Antibody (5F10) (CBMAB-C7535-LY)

This product is antibody recognizes ATP2B1. The antibody 5F10 immunoassay techniques such as: WB, IP, ELISA, IHC-P, IHC-F, FC, Inhibition, ICC/IF.
See all ATP2B1 antibodies
Published Data
Mouse Anti-ATP2B1 Recombinant Antibody (5F10) (CBMAB-C7535-LY) in WB
DRM fraction 3 from mouse brain preparations is enriched in cholesterol and sphingomyelin (n=6 mice; 3 males and 3 females; average±SEM shown; no diference between sex was detected). The membrane proteins Flot1, Cav1, Lamp2 and PMCA also appear in fraction 3, but show distinct and variable mobility in the sucrose gradient. ...View More
Corraliza-Gomez, M., del Caño-Espinel, M., Sanchez, D., & Ganfornina, M. D. (2022). The neuroprotective lipocalin apolipoprotein D stably interacts with specific subtypes of detergent-resistant membrane domains in a basigin-independent manner. Molecular Neurobiology, 59(7), 4015-4029.
Mouse Anti-ATP2B1 Recombinant Antibody (5F10) (CBMAB-C7535-LY) in WB
Protein levels for PMCA and OPN in hepatocytes and AS-30D cells were detected by Western blot. One hundred micrograms of protein (first lane) and 200 mg (second lane) were run for each cell type. ...View More
Briones-Orta, M. A., Delgado-Coello, B., Gutiérrez-Vidal, R., Sosa-Garrocho, M., Macías-Silva, M., & Mas-Oliva, J. (2021). Quantitative Expression of Key Cancer Markers in the AS-30D Hepatocarcinoma Model. Frontiers in Oncology, 11, 670292.
Mouse Anti-ATP2B1 Recombinant Antibody (5F10) (CBMAB-C7535-LY) in WB
Expression of Ca2+ signaling molecules in WT and RGS4−/−cells. Western blotting was performed using anti-SERCA2b, anti-PMCA (5F10), anti-IP3R2, and anti-IP3R3 antibodies in isolated pancreatic acinar cells. ...View More
Park, S., Lee, S. I., & Shin, D. M. (2011). Role of regulators of g-protein signaling 4 in ca signaling in mouse pancreatic acinar cells.

Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Cattle, Dog, Human
Clone
5F10
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 719-738 of PMCA1/4 of human origin.
Specificity
Mouse, Rat, Cattle, Dog, Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATPase, Ca++ transporting, plasma membrane 1
Introduction
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human490
Mouse67972
Rat29598
Sheep101111268
Rabbit100009543
Chicken374244
Hamster100764066
Cattle282641
Dog403480
UniProt ID
HumanP20020
MouseG5E829
RatP11505
SheepW5Q289
RabbitQ00804
ChickenQ98SH2
HamsterA0A1U8BUY0
CattleA0A3Q1ND98
DogE2R7S1
Function
Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity).
Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511).
May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.
Biological Process
Aging Source: Ensembl
Brain development Source: Ensembl
Calcium ion export across plasma membrane Source: SynGO-UCL
Cellular calcium ion homeostasis Source: GO_Central
Cellular response to corticosterone stimulus Source: Ensembl
Cellular response to vitamin D Source: Ensembl
Ion transmembrane transport Source: Reactome
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of cytosolic calcium ion concentration Source: UniProtKB
Neural retina development Source: Ensembl
Positive regulation of bone mineralization Source: UniProtKB
Positive regulation of calcium ion transport Source: UniProtKB
Regulation of blood pressure Source: UniProtKB
Regulation of cardiac conduction Source: Reactome
Regulation of cellular response to insulin stimulus Source: UniProtKB
Regulation of cytosolic calcium ion concentration Source: SynGO-UCL
Regulation of vascular associated smooth muscle contraction Source: UniProtKB
Response to cold Source: Ensembl
Cellular Location
Cell membrane; Basolateral cell membrane; Presynaptic cell membrane; Synapse; Synaptic vesicle membrane. Colocalizes with SV2A in photoreceptor synaptic terminals. Colocalizes with NPTN to the immunological synapse. Colocalizes with EPB41 to the basolateral membrane in enterocyte. Preferentially sorted to recycling synaptic vesicles.
Topology
Cytoplasmic: 2-105 aa
Helical: 106-126 aa
Extracellular: 127-154 aa
Helical: 155-175 aa
Cytoplasmic: 176-366 aa
Helical: 367-386 aa
Extracellular: 387-418 aa
Helical: 419-439 aa
Cytoplasmic: 440-855 aa
Helical: 856-876 aa
Extracellular: 877-882 aa
Helical: 883-903 aa
Cytoplasmic: 904-927 aa
Helical: 928-948 aa
Extracellular: 949-971 aa
Helical: 972-991 aa
Cytoplasmic: 992-1005 aa
Helical: 1006-1027 aa
Extracellular: 1028-1039 aa
Helical: 1040-1060 aa
Cytoplasmic: 1061-1220 aa

Zhu, L., & Liu, Z. (2021). Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR‑27b‑3p/ATPase plasma membrane Ca 2+ transporting 1 axis. Molecular Medicine Reports, 23(5), 1-10.

Korthals, M., Tech, L., Langnaese, K., Gottfried, A., Hradsky, J., Thomas, U., ... & Fischer, K. D. (2021). Plasma membrane Ca2+ ATPase 1 (PMCA1) but not PMCA4 is critical for B‐cell development and Ca2+ homeostasis in mice. European Journal of Immunology, 51(3), 594-602.

Althwab, S. A., Ahmed, A. A., Rasheed, Z., Alkhowailed, M., Hershan, A., Alsagaby, S., ... & Abdulmonem, W. A. (2021). ATP2B1 genotypes rs2070759 and rs2681472 polymorphisms and risk of hypertension in Saudi population. Nucleosides, Nucleotides & Nucleic Acids, 1-15.

Song, K. Y., Zhang, X. Z., Li, F., & Ji, Q. R. (2020). Silencing of ATP2B1‐AS1 contributes to protection against myocardial infarction in mouse via blocking NFKBIA‐mediated NF‐κB signalling pathway. Journal of cellular and molecular medicine, 24(8), 4466-4479.

Sun, X. M., Yang, M., & Jiang, C. X. (2019). Association of ATP2B1 gene polymorphism with incidence of eclampsia. Eur Rev Med Pharmacol Sci, 23(24), 10609-10616.

Horecka, E., Horecky, C., Kovarikova, L., Musilova, A., Knoll, A., Nedomova, S., & Pavlik, A. (2018). Association between single nucleotide polymorphisms of ATP2B1 gene and bone parameters of laying hens. Avian Biology Research, 11(3), 178-182.

Long, Y., Chen, S. W., Gao, C. L., He, X. M., Liang, G. N., Wu, J., ... & Chen, F. (2018). ATP2B1 gene silencing increases NO production under basal conditions through the Ca 2+/calmodulin/eNOS signaling pathway in endothelial cells. Hypertension Research, 41(4), 246-252.

Long, Y., Xia, J. Y., Chen, S. W., Gao, C. L., Liang, G. N., He, X. M., ... & Xu, Y. (2017). ATP2B1 gene silencing increases insulin sensitivity through facilitating Akt activation via the Ca2+/calmodulin signaling pathway and Ca2+-associated eNOS activation in endothelial cells. International journal of biological sciences, 13(9), 1203.

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For research use only. Not intended for any clinical use.

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