Human ATP2B1 ELISA Kit (V2LY-0626-LY5035)

Name: Human ATP2B1 ELISA Kit
SKU: V2LY-0626-LY5035
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0092 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

134.7 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

ATPase, Ca++ transporting, plasma membrane 1

Function

Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity).
Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511).
May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.

Biological Process

Aging Source: Ensembl
Brain development Source: Ensembl
Calcium ion export across plasma membrane Source: SynGO-UCL
Cellular calcium ion homeostasis Source: GO_Central
Cellular response to corticosterone stimulus Source: Ensembl
Cellular response to vitamin D Source: Ensembl
Ion transmembrane transport Source: Reactome
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of cytosolic calcium ion concentration Source: UniProtKB
Neural retina development Source: Ensembl
Positive regulation of bone mineralization Source: UniProtKB
Positive regulation of calcium ion transport Source: UniProtKB
Regulation of blood pressure Source: UniProtKB
Regulation of cardiac conduction Source: Reactome
Regulation of cellular response to insulin stimulus Source: UniProtKB
Regulation of cytosolic calcium ion concentration Source: SynGO-UCL
Regulation of vascular associated smooth muscle contraction Source: UniProtKB
Response to cold Source: Ensembl

Cellular Location

Cell membrane; Basolateral cell membrane; Presynaptic cell membrane; Synapse; Synaptic vesicle membrane. Colocalizes with SV2A in photoreceptor synaptic terminals. Colocalizes with NPTN to the immunological synapse. Colocalizes with EPB41 to the basolateral membrane in enterocyte. Preferentially sorted to recycling synaptic vesicles.

Topology

Cytoplasmic: 2-105 aa
Helical: 106-126 aa
Extracellular: 127-154 aa
Helical: 155-175 aa
Cytoplasmic: 176-366 aa
Helical: 367-386 aa
Extracellular: 387-418 aa
Helical: 419-439 aa
Cytoplasmic: 440-855 aa
Helical: 856-876 aa
Extracellular: 877-882 aa
Helical: 883-903 aa
Cytoplasmic: 904-927 aa
Helical: 928-948 aa
Extracellular: 949-971 aa
Helical: 972-991 aa
Cytoplasmic: 992-1005 aa
Helical: 1006-1027 aa
Extracellular: 1028-1039 aa
Helical: 1040-1060 aa
Cytoplasmic: 1061-1220 aa

Zhu, L., & Liu, Z. (2021). Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR‑27b‑3p/ATPase plasma membrane Ca 2+ transporting 1 axis. Molecular Medicine Reports, 23(5), 1-10.

Korthals, M., Tech, L., Langnaese, K., Gottfried, A., Hradsky, J., Thomas, U., ... & Fischer, K. D. (2021). Plasma membrane Ca2+ ATPase 1 (PMCA1) but not PMCA4 is critical for B‐cell development and Ca2+ homeostasis in mice. European Journal of Immunology, 51(3), 594-602.

Althwab, S. A., Ahmed, A. A., Rasheed, Z., Alkhowailed, M., Hershan, A., Alsagaby, S., ... & Abdulmonem, W. A. (2021). ATP2B1 genotypes rs2070759 and rs2681472 polymorphisms and risk of hypertension in Saudi population. Nucleosides, Nucleotides & Nucleic Acids, 1-15.

Song, K. Y., Zhang, X. Z., Li, F., & Ji, Q. R. (2020). Silencing of ATP2B1‐AS1 contributes to protection against myocardial infarction in mouse via blocking NFKBIA‐mediated NF‐κB signalling pathway. Journal of cellular and molecular medicine, 24(8), 4466-4479.

Sun, X. M., Yang, M., & Jiang, C. X. (2019). Association of ATP2B1 gene polymorphism with incidence of eclampsia. Eur Rev Med Pharmacol Sci, 23(24), 10609-10616.

Horecka, E., Horecky, C., Kovarikova, L., Musilova, A., Knoll, A., Nedomova, S., & Pavlik, A. (2018). Association between single nucleotide polymorphisms of ATP2B1 gene and bone parameters of laying hens. Avian Biology Research, 11(3), 178-182.

Long, Y., Chen, S. W., Gao, C. L., He, X. M., Liang, G. N., Wu, J., ... & Chen, F. (2018). ATP2B1 gene silencing increases NO production under basal conditions through the Ca 2+/calmodulin/eNOS signaling pathway in endothelial cells. Hypertension Research, 41(4), 246-252.

Long, Y., Xia, J. Y., Chen, S. W., Gao, C. L., Liang, G. N., He, X. M., ... & Xu, Y. (2017). ATP2B1 gene silencing increases insulin sensitivity through facilitating Akt activation via the Ca2+/calmodulin signaling pathway and Ca2+-associated eNOS activation in endothelial cells. International journal of biological sciences, 13(9), 1203.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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