Rabbit Anti-ATP5F1B Recombinant Antibody (
5F10) (V2LY-0725-LY1056)

Name: Rabbit Anti-ATP5F1B Recombinant Antibody (5F10)
SKU: V2LY-0725-LY1056
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ATP5F1B Recombinant Antibody (5F10) (V2LY-0725-LY1056) in WB

Western Blot
Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue
All lanes: ATP5F1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

Rabbit Anti-ATP5F1B Recombinant Antibody (5F10) (V2LY-0725-LY1056) in IHC

IHC image of V2LY-0725-LY1056 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ATP5F1B Recombinant Antibody (5F10) (V2LY-0725-LY1056) in IHC

IHC image of V2LY-0725-LY1056 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

5F10

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human ATPB.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATP Synthase F1 Subunit Beta

Entrez Gene ID

506

UniProt ID

P06576

Function

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Biological Process

Angiogenesis Source: UniProtKB
ATP biosynthetic process Source: UniProtKB
Cellular response to interleukin-7 Source: Ensembl
Cristae formation Source: Reactome
Generation of precursor metabolites and energy Source: UniProtKB
Lipid metabolic process Source: Ensembl
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Mitochondrion organization Source: Reactome
Negative regulation of cell adhesion involved in substrate-bound cell migration Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Positive regulation of blood vessel endothelial cell migration Source: CAFA
Proton transmembrane transport Source: UniProtKB
Regulation of intracellular pH Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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