Mouse Anti-ATP6V1B1 Recombinant Antibody (3G11) (CBMAB-A4089-YC)

Name: Mouse Anti-ATP6V1B1 Recombinant Antibody (3G11)
SKU: CBMAB-A4089-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3G11

Application

ELISA

Immunogen

Partial recombinant corresponding to aa 1-75 from human ATP6V1B1 (NP_001683. 2) with GST tag.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATPase H+ Transporting V1 Subunit B1

Introduction

ATP6V1B1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, z

Entrez Gene ID

525

UniProt ID

P15313

Alternative Names

ATPase H+ Transporting V1 Subunit B1; ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1; Endomembrane Proton Pump 58 KDa Subunit; Vacuolar Proton Pump Subunit B 1; Vacuolar Proton Pump 3; V-ATPase Subunit B 1; ATP6B1; VPP3; VATB;

Function

Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16769747).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
Essential for the proper assembly and activity of V-ATPase (PubMed:16769747).
In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16769747).
Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity).

Biological Process

Adult behavior Source: Ensembl
ATP metabolic process Source: Ensembl
Calcium ion homeostasis Source: UniProtKB
Cellular response to amino acid starvation Source: Reactome
Chloride ion homeostasis Source: Ensembl
Excretion Source: UniProtKB
Inner ear morphogenesis Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Olfactory behavior Source: Ensembl
Ossification Source: HGNC-UCL
Phagosome acidification Source: Reactome
pH reduction Source: UniProtKB
Potassium ion homeostasis Source: Ensembl
Prostaglandin metabolic process Source: Ensembl
Proton transmembrane transport Source: HGNC-UCL
Regulation of gene expression Source: Ensembl
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of pH Source: HGNC-UCL
Renal sodium excretion Source: Ensembl
Renal sodium ion transport Source: Ensembl
Renal water homeostasis Source: Ensembl
Sensory perception of sound Source: UniProtKB
Transferrin transport Source: Reactome

Cellular Location

Apical cell membrane; Basolateral cell membrane

Involvement in disease

Renal tubular acidosis, distal, with progressive nerve deafness (dRTA-D): An autosomal recessive disease characterized by the association of renal distal tubular acidosis with sensorineural hearing loss. Distal renal tubular acidosis is characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. It is due to functional failure of alpha-intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification.

Jamalpoor, A., van Eerde, A., Lilien, M. R., van Gelder, C. A., Zaal, E. A., Valentijn, F. A., ... & Janssen, M. J. (2020). The lysosomal V-ATPase B1 subunit in proximal tubule is linked to nephropathic cystinosis. bioRxiv.

Frische, S., Chambrey, R., Trepiccione, F., Zamani, R., Marcussen, N., Alexander, R. T., ... & Dimke, H. (2018). Molecular Mechanism of Renal Tubule Transport: H+-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney. American Journal of Physiology-Renal Physiology, 315(3), F429.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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