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Rabbit Anti-ATP6V1B1 Recombinant Antibody (EG308) (CBMAB-EN339-LY)

The product is antibody recognizes ATP6V1B1 . The antibody EG308 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all ATP6V1B1 antibodies
Published Data
Rabbit Anti-ATP6V1B1 Recombinant Antibody (EG308) in IP
Representative coimmunoprecipitation of lysates from WT PSEN1-expressing or PSEN1 KO SH-SY5Y cells. First two lanes show the input lysate (5 μg). Immunoprecipitation was performed using anti-PSEN1 or anti-IgG antibody. Coimmunoprecipitation was confirmed using antibody against TPCN2, MCOLN1, ATP6V1B1, or PSEN1. ...View More
Tong, B. C. K., Wu, A. J., Huang, A. S., Dong, R., Malampati, S., Iyaswamy, A., ... & Cheung, K. H. (2022). Lysosomal TPCN (two pore segment channel) inhibition ameliorates beta-amyloid pathology and mitigates memory impairment in Alzheimer disease. Autophagy, 18(3), 624-642.
Rabbit Anti-ATP6V1B1 Recombinant Antibody (EG308) in IF
Immunofluorescence analyses of day 20 organoids derived from GFP+ NPs and GFP UB cells show GFPAQP2+GATA3+, GFPAQP3+GATA3+, and GFPAQP4+GATA3+ principal cells and GFPPENDRIN+GATA3+ and GFPATP6V1B1+GATA3+ intercalated cells of collecting ducts. Note that GFP+AQP2+GATA3, GFP+AQP4+GATA3, and GFP+PENDRIN+GATA3+ distal tubules were also found. ...View More
Tsujimoto, H., Kasahara, T., Sueta, S. I., Araoka, T., Sakamoto, S., Okada, C., ... & Osafune, K. (2020). A modular differentiation system maps multiple human kidney lineages from pluripotent stem cells. Cell reports, 31(1).

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG308
Antibody Isotype
IgG
Application
WB, IHC-P

Basic Information

Immunogen
Synthetic peptide.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:10,000
IHC-P1:250-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 40% Glycerol, 0.05% BSA
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATPase H+ Transporting V1 Subunit B1
Introduction
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human525
Mouse110935
UniProt ID
HumanP15313
MouseQ66JS0
Alternative Names
ATPase H+ Transporting V1 Subunit B1; ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1; Endomembrane Proton Pump 58 KDa Subunit; Vacuolar Proton Pump Subunit B 1; Vacuolar Proton Pump 3; V-ATPase Subunit B 1; ATP6B1; VPP3; VATB;
Function
Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16769747).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
Essential for the proper assembly and activity of V-ATPase (PubMed:16769747).
In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16769747).
Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (By similarity).
Biological Process
Adult behavior Source: Ensembl
ATP metabolic process Source: Ensembl
Calcium ion homeostasis Source: UniProtKB
Cellular response to amino acid starvation Source: Reactome
Chloride ion homeostasis Source: Ensembl
Excretion Source: UniProtKB
Inner ear morphogenesis Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Olfactory behavior Source: Ensembl
Ossification Source: HGNC-UCL
Phagosome acidification Source: Reactome
pH reduction Source: UniProtKB
Potassium ion homeostasis Source: Ensembl
Prostaglandin metabolic process Source: Ensembl
Proton transmembrane transport Source: HGNC-UCL
Regulation of gene expression Source: Ensembl
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of pH Source: HGNC-UCL
Renal sodium excretion Source: Ensembl
Renal sodium ion transport Source: Ensembl
Renal water homeostasis Source: Ensembl
Sensory perception of sound Source: UniProtKB
Transferrin transport Source: Reactome
Cellular Location
Apical cell membrane; Basolateral cell membrane
Involvement in disease
Renal tubular acidosis, distal, with progressive nerve deafness (dRTA-D): An autosomal recessive disease characterized by the association of renal distal tubular acidosis with sensorineural hearing loss. Distal renal tubular acidosis is characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. It is due to functional failure of alpha-intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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