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Mouse Anti-B3GAT1 Recombinant Antibody (HNK-1) (CBMAB-G0007-LY)


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Published Data
Mouse Anti-B3GAT1 Recombinant Antibody (HNK-1) in Immunoblot
Immunoblot analysis comparing the expression of neural stemness (SOX2, ZEB1), EMT (TWIST1, SNAI1, SLUG/SNAI2, HNK1) and NCR and MSCrelated proteins (ITGB1/CD29; TFAP2A/AP2α) at neural stem cell (NSC) stage and across diferent passages of NCR cell culture. ...View More
Thomas, R., Menon, V., Mani, R., & Pruszak, J. (2022). Glycan epitope and integrin expression dynamics characterize neural crest epithelial-to-mesenchymal transition (EMT) in human pluripotent stem cell differentiation. Stem Cell Reviews and Reports, 18(8), 2952-2965.
Mouse Anti-B3GAT1 Recombinant Antibody (HNK-1) in ICC
Immunocytochemical analysis of diferent passages throughout NCR diferentiation (P0, P3, P6 and P9) for neural progenitors (DCX and SOX2), NCR (HNK1/CD57) and mesenchymal cells (CD44, Vimentin) confrms the presence of clearly distinct phenotypic stages in long-term NCR culture in congruence with morphological changes (Scale bar: 50 μm). ...View More
Thomas, R., Menon, V., Mani, R., & Pruszak, J. (2022). Glycan epitope and integrin expression dynamics characterize neural crest epithelial-to-mesenchymal transition (EMT) in human pluripotent stem cell differentiation. Stem Cell Reviews and Reports, 18(8), 2952-2965.
Mouse Anti-B3GAT1 Recombinant Antibody (HNK-1) in FC
Flow cytometric characterization of surface markers across four diferent stages of NCR diferentiation (Passages 0, 3, 6 and 9) shows the temporal changes in surface marker expression as the cells progress along NCR diferentiation. Error bars represent arithmetic mean±SD (n=3; CD90high at P9 n=2). ...View More
Thomas, R., Menon, V., Mani, R., & Pruszak, J. (2022). Glycan epitope and integrin expression dynamics characterize neural crest epithelial-to-mesenchymal transition (EMT) in human pluripotent stem cell differentiation. Stem Cell Reviews and Reports, 18(8), 2952-2965.
Mouse Anti-B3GAT1 Recombinant Antibody (HNK-1) in ICC
Immunocytochemistry analysis showed that H9 hESC-derived H9-NCCs were positive for HNK1, FOXD3, AP2α, p75, SOX9, SOX10 and were negative for neural progenitor marker PAX6 and undifferentiated hESCs markers
SOX2 and OCT4. ...View More
Liu, W., Wang, K., Lv, X., Wang, Q., Li, X., Yang, Z., ... & Xiao, R. (2020). Up-regulation of RNA binding proteins contributes to folate deficiency-induced neural crest cells dysfunction. International journal of biological sciences, 16(1), 85.

Summary

Host Animal
Mouse
Specificity
Human
Clone
HNK-1
Antibody Isotype
IgM, κ
Application
FC, IF, IHC-P, WB, IP

Basic Information

Immunogen
Peripheral blood mononuclear cells of human origin.
Specificity
Human
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Beta-1,3-Glucuronyltransferase 1
Introduction
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Entrez Gene ID
27087
UniProt ID
Q9P2W7
Alternative Names
Beta-1;3-Glucuronyltransferase 1; Glucuronosyltransferase P; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1; UDP-GlcUA:Glycoprotein Beta-1;3-Glucuronyltransferase; EC 2.4.1.135; GlcUAT-P; GLCATP; CD57 Antigen; LEU7 Antigen;
Function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Biological Process
Carbohydrate metabolic process Source: GO_Central
Cellular response to hypoxia Source: Ensembl
Chondroitin sulfate proteoglycan biosynthetic process Source: GO_Central
Glycosaminoglycan metabolic process Source: Reactome
Protein glycosylation Source: UniProtKB-UniPathway
Cellular Location
Isoform 1: Secreted; Golgi apparatus membrane
Isoform 2: Secreted; Golgi apparatus membrane; Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-6 aa
Helical: 7-27 aa
Lumenal: 28-334 aa
PTM
The soluble form derives from the membrane form by proteolytic processing.
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For research use only. Not intended for any clinical use.

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