Mouse Anti-B3GAT1 Recombinant Antibody (NK-1) (CBMAB-C5848-CQ)

Name: Mouse Anti-B3GAT1 Recombinant Antibody (NK-1)
SKU: CBMAB-C5848-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

NK-1

Application

FC, IF, IHC-P, WB, IP

Immunogen

Human peripheral blood mononuclear cells

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Beta-1,3-Glucuronyltransferase 1

Introduction

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.

Entrez Gene ID

27087

UniProt ID

Q9P2W7

Alternative Names

Beta-1,3-Glucuronyltransferase 1; Glucuronosyltransferase P; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1; UDP-GlcUA:Glycoprotein Beta-1,3-Glucuronyltransferase; EC 2.4.1.135; GlcUAT-P; GLCATP; CD57 Antigen; LEU7 Antigen;

Function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Biological Process

Carbohydrate metabolic process Source: GO_Central
Cellular response to hypoxia Source: Ensembl
Chondroitin sulfate proteoglycan biosynthetic process Source: GO_Central
Glycosaminoglycan metabolic process Source: Reactome
Protein glycosylation Source: UniProtKB-UniPathway

Cellular Location

Isoform 1: Secreted; Golgi apparatus membrane
Isoform 2: Secreted; Golgi apparatus membrane; Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-6 aa
Helical: 7-27 aa
Lumenal: 28-334 aa

PTM

The soluble form derives from the membrane form by proteolytic processing.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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