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Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166) (CBMAB-0167-YY)

This product is rabbit antibody that recognizes BAG5. The antibody CBYY-0166 can be used for immunoassay techniques such as: WB
See all BAG5 antibodies
Published Data
Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166) in WB
The OC cells (OVCAR3, OVCAR4 and Kuramochi) were transfected with pre-miR-4454 or scrambled control oligo (Scr) (left panel) or with BAG5 siRNA or scrambled control oligos (Scr) (right panel). Cells were allowed to grow for 48 h and then lysed in RIPA buffer. Proteins were separated by 4-20% gradient SDS-PAGE and transferred to nitrocellulose membrane, probed with BAG5, cleaved PARP, cleaved caspase-3 and p53 antibodies. Representative blots from 3 independent experiments are shown. ...View More
Dasari, S., Pandhiri, T., Grassi, T., Visscher, D. W., Multinu, F., Agarwal, K., ... & Mitra, A. K. (2020). Signals from the metastatic niche regulate early and advanced ovarian cancer metastasis through miR-4454 downregulation. Molecular Cancer Research, 18(8), 1202-1217.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0166
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of Bag-5 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
C-Terminus

Target

Full Name
BCL2 Associated Athanogene 5
Introduction
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforMouse have been found for this gene.
Entrez Gene ID
9529
UniProt ID
Q9UL15
Alternative Names
BCL2 Associated Athanogene 5; Bcl-2-Associated Athanogene 5; BAG-5; BAG Family Molecular Chaperone Regulator 5; KIAA0873;
Function
Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).
May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Biological Process
Golgi organization Source: ParkinsonsUK-UCL
Negative regulation of neuron projection development Source: ParkinsonsUK-UCL
Negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
Negative regulation of protein refolding Source: BHF-UCL
Negative regulation of protein ubiquitination Source: ARUK-UCL
Negative regulation of ubiquitin-protein transferase activity Source: BHF-UCL
Neuron death Source: BHF-UCL
Protein folding Source: ProtInc
Protein stabilization Source: ARUK-UCL
Regulation of cellular response to heat Source: Reactome
Regulation of inclusion body assembly Source: BHF-UCL
Regulation of ubiquitin-protein transferase activity Source: ParkinsonsUK-UCL
Cellular Location
Cytosol; Mitochondrion; Nucleus; Cytoplasm; Inclusion body; Membrane; Perinuclear region of cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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