Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166) (CBMAB-0167-YY)

Name: Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166)
SKU: CBMAB-0167-YY
Rating: 5 (1 reviews)

See all BAG5 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166) in WB

The OC cells (OVCAR3, OVCAR4 and Kuramochi) were transfected with pre-miR-4454 or scrambled control oligo (Scr) (left panel) or with BAG5 siRNA or scrambled control oligos (Scr) (right panel). Cells were allowed to grow for 48 h and then lysed in RIPA buffer. Proteins were separated by 4-20% gradient SDS-PAGE and transferred to nitrocellulose membrane, probed with BAG5, cleaved PARP, cleaved caspase-3 and p53 antibodies. Representative blots from 3 independent experiments are shown. ...View More

Dasari, S., Pandhiri, T., Grassi, T., Visscher, D. W., Multinu, F., Agarwal, K., ... & Mitra, A. K. (2020). Signals from the metastatic niche regulate early and advanced ovarian cancer metastasis through miR-4454 downregulation. Molecular Cancer Research, 18(8), 1202-1217.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0166

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of Bag-5 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

C-Terminus

More Infomation

Target

Full Name

BCL2 Associated Athanogene 5

Introduction

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforMouse have been found for this gene.

Entrez Gene ID

9529

UniProt ID

Q9UL15

Alternative Names

BCL2 Associated Athanogene 5; Bcl-2-Associated Athanogene 5; BAG-5; BAG Family Molecular Chaperone Regulator 5; KIAA0873;

Function

Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).
May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.

Biological Process

Golgi organization Source: ParkinsonsUK-UCL
Negative regulation of neuron projection development Source: ParkinsonsUK-UCL
Negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
Negative regulation of protein refolding Source: BHF-UCL
Negative regulation of protein ubiquitination Source: ARUK-UCL
Negative regulation of ubiquitin-protein transferase activity Source: BHF-UCL
Neuron death Source: BHF-UCL
Protein folding Source: ProtInc
Protein stabilization Source: ARUK-UCL
Regulation of cellular response to heat Source: Reactome
Regulation of inclusion body assembly Source: BHF-UCL
Regulation of ubiquitin-protein transferase activity Source: ParkinsonsUK-UCL

Cellular Location

Cytosol; Mitochondrion; Nucleus; Cytoplasm; Inclusion body; Membrane; Perinuclear region of cytoplasm

Wang, J. M., Gao, Q., Zhang, Q., Hao, L., Jiang, J. Y., Huyan, L. Y., ... & Wang, H. Q. (2021). Implication of BAG5 downregulation in metabolic reprogramming of cisplatin-resistant ovarian cancer cells via mTORC2 signaling pathway. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 119076.

Friesen, E. L., Zhang, Y. T., Earnshaw, R., De Snoo, M. L., O’Hara, D. M., Agapova, V., ... & Kalia, S. K. (2020). BAG5 Promotes Alpha-Synuclein Oligomer Formation and Functionally Interacts With the Autophagy Adaptor Protein p62. Frontiers in Cell and Developmental Biology, 8, 716.

Zhang, D. L., Wang, J. M., Wu, T., Du, X., Yan, J., Du, Z. X., & Wang, H. Q. (2020). BAG5 promotes invasion of papillary thyroid cancer cells via upregulation of fibronectin 1 at the translational level. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(9), 118715.

Chen, H. Y., Lin, C. H., & Teng, S. C. (2020). Stress-induced p53 drives BAG5 cochaperone expression to control α-synuclein aggregation in Parkinson's disease. Aging (Albany NY), 12(20), 20702.

De Snoo, M. L., Friesen, E. L., Zhang, Y. T., Earnshaw, R., Dorval, G., Kapadia, M., ... & Kalia, S. K. (2019). Bcl-2-associated athanogene 5 (BAG5) regulates Parkin-dependent mitophagy and cell death. Cell death & disease, 10(12), 1-14.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-BAG5 Recombinant Antibody (CBYY-0166)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

BAG5 Matched Antibody Pair (124) (CAT#: APMAB-124LY)

BAG5 Matched Antibody Pair (125) (CAT#: APMAB-125LY)

Related Products

BAG5 Matched Antibody Pair (124) (CAT#: APMAB-124LY)

BAG5 Matched Antibody Pair (125) (CAT#: APMAB-125LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry