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Mouse Anti-BCAT1 Recombinant Antibody (1F8) (CBMAB-1195-CN)

This product is a mouse antibody that recognizes BCAT1 of human. The antibody 1F8 can be used for immunoassay techniques such as: WB, ELISA.
See all BCAT1 antibodies
Published Data
Fig. 1 Western blot: BCAT1 protein level was significantly changed after different treatment.
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Fig. 2 Immunohistochemical staining of tumors showed the levels of BCAT1 in sh‐CRNDE group were lower than that of NC group.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1F8
Antibody Isotype
IgG2a
Application
WB, ELISA

Basic Information

Immunogen
BCAT1 (AAH33864, aa. 1-320) full-length recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Localized in aa. 1-320

Target

Full Name
branched chain aminotransferase 1, cytosolic
Introduction
This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. This protein catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Entrez Gene ID
586
UniProt ID
P54687
Alternative Names
BCT1; PP18; BCATC; ECA39; MECA39; PNAS121
Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Biological Process
Branched-chain amino acid biosynthetic process Source: ProtInc
Branched-chain amino acid catabolic process Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Leucine biosynthetic process Source: GO_Central
Valine biosynthetic process Source: GO_Central
Cellular Location
Cytoplasm

Yi, L., Fan, X., Li, J., Yuan, F., Zhao, J., Nistér, M., & Yang, X. (2021). Enrichment of branched chain amino acid transaminase 1 correlates with multiple biological processes and contributes to poor survival of IDH1 wild-type gliomas. Aging (Albany NY), 13(3), 3645.

Luo, L., Sun, W., Zhu, W., Li, S., Zhang, W., Xu, X., ... & Zheng, Y. (2021). BCAT1 decreases the sensitivity of cancer cells to cisplatin by regulating mTOR-mediated autophagy via branched-chain amino acid metabolism. Cell death & disease, 12(2), 1-13.

Francois Martín del Campo, L. (2021). Novel mode of action of the branched-chain amino acid transaminase BCAT1 in glioblastoma (Doctoral dissertation).

PLOS ONE Staff. (2019). Correction: Branched chain amino acid transaminase 1 (BCAT1) is overexpressed and hypomethylated in patients with non-alcoholic fatty liver disease who experience adverse clinical events: A pilot study. Plos one, 14(2), e0212144.

Grankvist, N., Lagerborg, K. A., Jain, M., & Nilsson, R. (2018). Gabapentin can suppress cell proliferation independent of the cytosolic branched-chain amino acid transferase 1 (BCAT1). Biochemistry, 57(49), 6762-6766.

Wegermann, K., Henao, R., Diehl, A. M., Murphy, S. K., Abdelmalek, M. F., & Moylan, C. A. (2018). Branched chain amino acid transaminase 1 (BCAT1) is overexpressed and hypomethylated in patients with non-alcoholic fatty liver disease who experience adverse clinical events: A pilot study. PloS one, 13(9), e0204308.

Zhang, L., & Han, J. (2017). Branched-chain amino acid transaminase 1 (BCAT1) promotes the growth of breast cancer cells through improving mTOR-mediated mitochondrial biogenesis and function. Biochemical and biophysical research communications, 486(2), 224-231.

Thewes, V., Simon, R., Hlevnjak, M., Schlotter, M., Schroeter, P., Schmidt, K., ... & Tönjes, M. (2017). The branched-chain amino acid transaminase 1 sustains growth of antiestrogen-resistant and ERα-negative breast cancer. Oncogene, 36(29), 4124-4134.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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