Mouse Anti-BCAT1 Recombinant Antibody (CBYY-0294) (CBMAB-0295-YY)

Name: Mouse Anti-BCAT1 Recombinant Antibody (CBYY-0294)
SKU: CBMAB-0295-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0294

Application

ELISA, WB

Immunogen

Recombinant human BCAT1 (1-386aa) purified from E. coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 10% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

branched chain aminotransferase 1, cytosolic

Introduction

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.

Entrez Gene ID

Human	586
Dog	486633
Mouse	12035

UniProt ID

Human	P54687
Dog	F1PY28
Mouse	P24288

Alternative Names

Branched Chain Amino Acid Transaminase 1; Branched Chain Aminotransferase 1, Cytosolic; EC 2.6.1.42; ECA39; BCT1; Branched-Chain-Amino-Acid Aminotransferase, Cytosolic; Branched Chain Amino-Acid Transaminase 1, Cytosolic; Placental Protein 18;

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Biological Process

Branched-chain amino acid biosynthetic process Source: ProtInc
Branched-chain amino acid catabolic process Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Leucine biosynthetic process Source: GO_Central
Valine biosynthetic process Source: GO_Central

Cellular Location

Cytoplasm

Yi, L., Fan, X., Li, J., Yuan, F., Zhao, J., Nistér, M., & Yang, X. (2021). Enrichment of branched chain amino acid transaminase 1 correlates with multiple biological processes and contributes to poor survival of IDH1 wild-type gliomas. Aging (Albany NY), 13(3), 3645.

Luo, L., Sun, W., Zhu, W., Li, S., Zhang, W., Xu, X., ... & Zheng, Y. (2021). BCAT1 decreases the sensitivity of cancer cells to cisplatin by regulating mTOR-mediated autophagy via branched-chain amino acid metabolism. Cell death & disease, 12(2), 1-13.

Francois Martín del Campo, L. (2021). Novel mode of action of the branched-chain amino acid transaminase BCAT1 in glioblastoma (Doctoral dissertation).

PLOS ONE Staff. (2019). Correction: Branched chain amino acid transaminase 1 (BCAT1) is overexpressed and hypomethylated in patients with non-alcoholic fatty liver disease who experience adverse clinical events: A pilot study. Plos one, 14(2), e0212144.

Grankvist, N., Lagerborg, K. A., Jain, M., & Nilsson, R. (2018). Gabapentin can suppress cell proliferation independent of the cytosolic branched-chain amino acid transferase 1 (BCAT1). Biochemistry, 57(49), 6762-6766.

Wegermann, K., Henao, R., Diehl, A. M., Murphy, S. K., Abdelmalek, M. F., & Moylan, C. A. (2018). Branched chain amino acid transaminase 1 (BCAT1) is overexpressed and hypomethylated in patients with non-alcoholic fatty liver disease who experience adverse clinical events: A pilot study. PloS one, 13(9), e0204308.

Zhang, L., & Han, J. (2017). Branched-chain amino acid transaminase 1 (BCAT1) promotes the growth of breast cancer cells through improving mTOR-mediated mitochondrial biogenesis and function. Biochemical and biophysical research communications, 486(2), 224-231.

Thewes, V., Simon, R., Hlevnjak, M., Schlotter, M., Schroeter, P., Schmidt, K., ... & Tönjes, M. (2017). The branched-chain amino acid transaminase 1 sustains growth of antiestrogen-resistant and ERα-negative breast cancer. Oncogene, 36(29), 4124-4134.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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