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Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC)

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Published Data
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in WB
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in WB
Verifcation of the results of MS experiments. Recombinant FGF12-His was bound to Ni Sepharose and incubated with U2OS cells lysate. FGF12-bound proteins were eluted and analyzed by wester blotting using specifc antibodies. ...View More
Sochacka, M., Karelus, R., Opalinski, L., Krowarsch, D., Biadun, M., Otlewski, J., & Zakrzewska, M. (2022). FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex. Cell Communication and Signaling, 20(1), 182.
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IF
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IF
Co-localization of FGF12-mGFP-myc with identifed partner protein. To analyze the co-localization of FGF12-mGFP-myc with NOLC1, TCOF1, BCLAF1, THRAP3, U2OS-FGF12-mGFP-myc cells were fxed, permeabilized and stained with appropriate primary antibodies and Alexa Fluor 594-conjugated anti-mouse secondary antibody. Cell nuclei were labeled with NucBlue Live and cells were analyzed by fuorescence microscopy. The scale bar represents 20 μm. ...View More
Sochacka, M., Karelus, R., Opalinski, L., Krowarsch, D., Biadun, M., Otlewski, J., & Zakrzewska, M. (2022). FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex. Cell Communication and Signaling, 20(1), 182.
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in WB
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in WB
HepG2 cells treated with NC-siRNA, NC-siRNA plus STA9090, Bclaf1-siRNA, and Bclaf1-siRNA plus STA9090 or treated with NC-siRNA, NC-siRNA plus STA9090, VPS35-siRNA, and VPS35-siRNA plus STA9090 for 48 h. The Bclaf1 and VPS35 levels were detected using western blotting. ...View More
Tan, W., Zhang, J., Liu, L., Liang, M., Li, J., Deng, Z., ... & Chen, X. (2022). Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma. Translational Oncology, 26, 101502.
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IF
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IF
Confocal immunofluorescence images showed VPS35 (red) and Bclaf1 (green) intensity. ...View More
Tan, W., Zhang, J., Liu, L., Liang, M., Li, J., Deng, Z., ... & Chen, X. (2022). Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma. Translational Oncology, 26, 101502.
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IHC
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IHC
Protein levels of Bclaf1 and VPS35 in Control, STA9090, and NB-treated tumors were detected by immunohistochemistry. Image Pro-plus 6.0 software was used to quantify the Mean of IOD and Value represent mean ± SD, n = 6 *P < 0.01, **P<0.01, ***P< 0.001. ...View More
Tan, W., Zhang, J., Liu, L., Liang, M., Li, J., Deng, Z., ... & Chen, X. (2022). Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma. Translational Oncology, 26, 101502.
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IHC
Mouse Anti-BCLAF1 Recombinant Antibody (M33-P5B11) (CBMAB-XB0089-YC) in IHC
The protein expression of Bclaf1 and VPS35 in tumor tissue (TT) or adjacent tissue (AT) of patients with HCC was detected by IHC. ...View More
Tan, W., Zhang, J., Liu, L., Liang, M., Li, J., Deng, Z., ... & Chen, X. (2022). Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma. Translational Oncology, 26, 101502.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
M33-P5B11
Application
ELISA, IP, WB
Immunogen
Synthetic peptide corresponding to amino acids 900-909 of BCLAF1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
BCL2 Associated Transcription Factor 1
Introduction
BCLAF1 is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis.
Entrez Gene ID
9774
UniProt ID
Q9NYF8
Alternative Names
BCL2 Associated Transcription Factor 1; BCLAF1 And THRAP3 Family Member 1; BTF; Bcl-2-Associated Transcription Factor 1; BCL2-Associated Transcription Factor 1; BK211L9.1; KIAA0164;
Function
Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Biological Process
Apoptotic process Source: UniProtKB
Cellular response to leukemia inhibitory factor Source: Ensembl
Negative regulation of transcription, DNA-templated Source: MGI
Positive regulation of apoptotic process Source: MGI
Positive regulation of DNA-templated transcription, initiation Source: UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of response to DNA damage stimulus Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of DNA-templated transcription in response to stress Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Nucleus speckle; Nucleoplasm
PTM
Citrullinated by PADI4.

Liu, J., Li, J., Sun, Z., Duan, Y., Wang, F., Wei, G., & Yang, J. H. (2021). Bcl-2-associated transcription factor 1 Ser290 phosphorylation mediates DNA damage response and regulates radiosensitivity in gastric cancer. Journal of Translational Medicine, 19(1), 1-16.

Zhang, Y., Zhang, X., Cai, B., Li, Y., Jiang, Y., Fu, X., ... & Pan, Z. (2021). The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury. Nature communications, 12(1), 1-15.

Mwapagha, L. M., Chibanga, V., Shipanga, H., & Parker, M. I. (2021). New insights from Whole Genome Sequencing: BCLAF1 deletion as a structural variant that predisposes cells towards cellular transformation. Oncology Reports, 46(4), 1-11.

Zhang, S., Zhang, M., Chen, J., Zhao, J., Su, J., & Zhang, X. (2020). Ginsenoside Compound K Regulates HIF-1α-Mediated Glycolysis Through Bclaf1 to Inhibit the Proliferation of Human Liver Cancer Cells. Frontiers in Pharmacology, 11, 2050.

Jiang, T., Liu, B., Wu, D., & Zhang, F. (2020). BCLAF1 induces cisplatin resistance in lung cancer cells. Oncology Letters, 20(5), 1-1.

Mou, S. J., Yang, P. F., Liu, Y. P., Xu, N., Jiang, W. W., & Yue, W. J. (2020). BCLAF1 promotes cell proliferation, invasion and drug-resistance though targeting lncRNA NEAT1 in hepatocellular carcinoma. Life sciences, 242, 117177.

Zhou, X., Wen, Y., Tian, Y., He, M., Ke, X., Huang, Z., ... & Zou, F. (2019). Heat shock protein 90α–dependent B‐cell‐2–associated transcription factor 1 promotes hepatocellular carcinoma proliferation by regulating MYC proto‐oncogene c‐MYC mRNA stability. Hepatology, 69(4), 1564-1581.

Qin, C., Zhang, R., Lang, Y., Shao, A., Xu, A., Feng, W., ... & Tang, J. (2019). Bclaf1 critically regulates the type I interferon response and is degraded by alphaherpesvirus US3. PLoS pathogens, 15(1), e1007559.

Dell'Aversana, C., Giorgio, C., D'amato, L., Lania, G., Matarese, F., Saeed, S., ... & Altucci, L. (2017). miR-194-5p/BCLAF1 deregulation in AML tumorigenesis. Leukemia, 31(11), 2315-2325.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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