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Mouse Anti-BCLAF1 Recombinant Antibody (2F2) (CBMAB-AP604LY)


See all BCLAF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F2
Antibody Isotype
IgG1
Application
IP, WB

Basic Information

Immunogen
E. coli-derived recombinant protein of amino acids 501-920 of human BCLAF1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BCL2 Associated Transcription Factor 1
Introduction
This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
9774
UniProt ID
Q9NYF8
Alternative Names
BCL2 Associated Transcription Factor 1; BCLAF1 And THRAP3 Family Member 1; BTF; Bcl-2-Associated Transcription Factor 1; BCL2-Associated Transcription Factor 1; BK211L9.1; KIAA0164;
Function
Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Biological Process
Apoptotic process Source: UniProtKB
Cellular response to leukemia inhibitory factor Source: Ensembl
Negative regulation of transcription, DNA-templated Source: MGI
Positive regulation of apoptotic process Source: MGI
Positive regulation of DNA-templated transcription, initiation Source: UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of response to DNA damage stimulus Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of DNA-templated transcription in response to stress Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Nucleus speckle; Nucleoplasm
PTM
Citrullinated by PADI4.

Liu, J., Li, J., Sun, Z., Duan, Y., Wang, F., Wei, G., & Yang, J. H. (2021). Bcl-2-associated transcription factor 1 Ser290 phosphorylation mediates DNA damage response and regulates radiosensitivity in gastric cancer. Journal of Translational Medicine, 19(1), 1-16.

Zhang, Y., Zhang, X., Cai, B., Li, Y., Jiang, Y., Fu, X., ... & Pan, Z. (2021). The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury. Nature communications, 12(1), 1-15.

Mwapagha, L. M., Chibanga, V., Shipanga, H., & Parker, M. I. (2021). New insights from Whole Genome Sequencing: BCLAF1 deletion as a structural variant that predisposes cells towards cellular transformation. Oncology Reports, 46(4), 1-11.

Zhang, S., Zhang, M., Chen, J., Zhao, J., Su, J., & Zhang, X. (2020). Ginsenoside Compound K Regulates HIF-1α-Mediated Glycolysis Through Bclaf1 to Inhibit the Proliferation of Human Liver Cancer Cells. Frontiers in Pharmacology, 11, 2050.

Jiang, T., Liu, B., Wu, D., & Zhang, F. (2020). BCLAF1 induces cisplatin resistance in lung cancer cells. Oncology Letters, 20(5), 1-1.

Mou, S. J., Yang, P. F., Liu, Y. P., Xu, N., Jiang, W. W., & Yue, W. J. (2020). BCLAF1 promotes cell proliferation, invasion and drug-resistance though targeting lncRNA NEAT1 in hepatocellular carcinoma. Life sciences, 242, 117177.

Zhou, X., Wen, Y., Tian, Y., He, M., Ke, X., Huang, Z., ... & Zou, F. (2019). Heat shock protein 90α–dependent B‐cell‐2–associated transcription factor 1 promotes hepatocellular carcinoma proliferation by regulating MYC proto‐oncogene c‐MYC mRNA stability. Hepatology, 69(4), 1564-1581.

Qin, C., Zhang, R., Lang, Y., Shao, A., Xu, A., Feng, W., ... & Tang, J. (2019). Bclaf1 critically regulates the type I interferon response and is degraded by alphaherpesvirus US3. PLoS pathogens, 15(1), e1007559.

Dell'Aversana, C., Giorgio, C., D'amato, L., Lania, G., Matarese, F., Saeed, S., ... & Altucci, L. (2017). miR-194-5p/BCLAF1 deregulation in AML tumorigenesis. Leukemia, 31(11), 2315-2325.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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