Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY)

Name: Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531)
SKU: CBMAB-0534-YY
Rating: 5 (1 reviews)

See all BHLHA15 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY) in WB

Western blot of IECs from Ptpn2fl/fl and Ptpn2DIEC mice probed for lysozyme-1, Mist1, and unfolded protein response marker Xbp-1s. ...View More

Canale, V., Spalinger, M. R., Alvarez, R., Sayoc-Becerra, A., Sanati, G., Manz, S., ... & McCole, D. F. (2023). PTPN2 is a critical regulator of ileal Paneth cell viability and function in mice. Cellular and Molecular Gastroenterology and Hepatology, 16(1), 39-62.

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY) in WB

Western blot of ileal IECs probing for Mist1 protein. ...View More

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY) in IF

Immunofuorescence staining of cells prepared as rat SMG tissue (positive control) was used to evaluate the presence and localization of amylase, Aqp5, Mist1, Krt14, Cldn3, and Cldn10 protein. Staining with nonspecifc isotype antibody was used as a negative control (not shown). Bar: 50 µm. ...View More

Tran, O. N., Wang, H., Li, S., Malakhov, A., Sun, Y., Abdul Azees, P. A., ... & Chen, X. D. (2022). Organ-specific extracellular matrix directs trans-differentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo. Stem Cell Research & Therapy, 13(1), 1-20.

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY) in Immunoblot

Tissue lysates from gastric mucosa were used to detect the transcription factor Mist1 (22 kDa) and pepsinogen C (42 kDa) at 18, and 60 days. Protein levels were determined by integrate optical densitometry (arbitrary units) after immunoblots and compared to the internal control protein (β-actin, 42 kDa). ...View More

Teles Silva, M., Mesquita da Silva, K., Campos Rattes, I., Maria Agostini Zonta, G., Vasques da Costa, A., Galvao Figueredo Costa, R., ... & Gama, P. (2019). Immediate and late effects of early weaning on rat gastric cell differentiation. International Journal of Molecular Sciences, 21(1), 196.

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531) (CBMAB-0534-YY) in IF

Representative photomicrographs of the gastric mucosa illustrate the distribution of mucous neck cells (MNC: +GSII/FITC), transition cell (TC: +GSII/FITC+Mist1/Cy3 or +GSII/FITC +Mist1/Cy3+GIF/AlexaFluor
633) and zymogenic cells (ZC: +Mist1/Cy3). Nuclei were counterstained with DAPI. Representative images were acquired using confocal microscopy (Zeiss LSM 780-NLO). Scale bar = 50 µm. The insets show in detail the morphology and labeling of each cell type; digital zoom was set at 57%. ...View More

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0531

Application

WB, IF, IP, ELISA, IHC-P

Immunogen

Amino acids 175-197 corresponding to the C-terminus of MIST1 of mouse origin.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

C-Terminus

More Infomation

Target

Full Name

basic helix-loop-helix family, member a15

Introduction

Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-Bovinex occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-Bovinex consensus sequence 5-CANNTG-3 (By similarity).

Entrez Gene ID

168620

UniProt ID

Q7RTS1

Alternative Names

Basic Helix-Loop-Helix Family Member A15; Basic Helix-Loop-Helix Domain Containing, Class B, 8; Muscle, Intestine And Stomach Expression 1; Class B Basic Helix-Loop-Helix Protein 8; BHLHB8; MIST-1;

Function

Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).

Biological Process

Calcium-mediated signaling Source: Ensembl
Cell-cell signaling Source: Ensembl
Cell maturation Source: Ensembl
Cellular response to glucose starvation Source: UniProtKB
Endoplasmic reticulum unfolded protein response Source: UniProtKB
Glucose homeostasis Source: Ensembl
Golgi organization Source: Ensembl
G protein-coupled receptor signaling pathway Source: Ensembl
Intracellular distribution of mitochondria Source: Ensembl
Mitochondrial calcium ion transmembrane transport Source: Ensembl
Negative regulation of myotube differentiation Source: UniProtKB
Neuron differentiation Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0531)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-BHLHA15 Recombinant Antibody (1E7) (CAT#: CBMAB-A0758-LY)

Rabbit Anti-BHLHA15 Recombinant Antibody (CBFYM-0881) (CAT#: CBMAB-M1028-FY)

Mouse Anti-BHLHA15 Recombinant Antibody (CBYY-0532) (CAT#: CBMAB-0535-YY)

Mouse Anti-BHLHA15 Recombinant Antibody (CBFYM-0880) (CAT#: CBMAB-M1027-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry