Mouse Anti-BirA Recombinant Antibody (5B11c3-3) (CBMAB-AP638LY)

Name: Mouse Anti-BirA Recombinant Antibody (5B11c3-3)
SKU: CBMAB-AP638LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5B11c3-3

Application

IF, WB

Immunogen

Amino acids residues 151-321 of E.coli BirA.

Host Species

Mouse

Specificity

E.coli

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BirA

Function

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.

Biological Process

Biotin biosynthetic process Source: EcoliWiki
Biotin metabolic process Source: CAFA
Protein biotinylation Source: CACAO
Regulation of transcription, DNA-templated Source: UniProtKB-UniRule

Cellular Location

Cytoplasm; Transcription repressor complex

Satiaputra, J., Sternicki, L. M., Hayes, A. J., Pukala, T. L., Booker, G. W., Shearwin, K. E., & Polyak, S. W. (2019). Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus. Scientific reports, 9(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-BirA Recombinant Antibody (6C4c7) (CAT#: CBMAB-AP639LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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