E. coli Recombinant BirA protein, His & MBP Tag (V2LY-0526-LY1772)

Name: E. coli Recombinant BirA protein, His & MBP Tag
SKU: V2LY-0526-LY1772
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

E. coli

Tag

His & MBP Tag

Protein Construction

This product is E. coli Recombinant BirA protein, His & MBP Tag consist of Amino Acid: 1-321 and predicts a molecular mass of 78.3 kDa.

Molecule Mass

78.3 kDa

Sequence

Amino Acid: 1-321

Species

E. coli

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

BirA

Function

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.

Biological Process

Biotin biosynthetic process Source: EcoliWiki
Biotin metabolic process Source: CAFA
Protein biotinylation Source: CACAO
Regulation of transcription, DNA-templated Source: UniProtKB-UniRule

Cellular Location

Cytoplasm; Transcription repressor complex

Satiaputra, J., Sternicki, L. M., Hayes, A. J., Pukala, T. L., Booker, G. W., Shearwin, K. E., & Polyak, S. W. (2019). Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus. Scientific reports, 9(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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