Mouse Anti-BLMH Recombinant Antibody (CBYY-2075) (CBMAB-2424-YY)

Name: Mouse Anti-BLMH Recombinant Antibody (CBYY-2075)
SKU: CBMAB-2424-YY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-2075

Antibody Isotype

IgG1, κ

Application

E, WB, IP, IF, P

Basic Information

Immunogen

Amino acids 1-300 mapping at the N-terminus of BLM hydrolase of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Bleomycin Hydrolase

Introduction

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.

Entrez Gene ID

642

UniProt ID

Q13867

Alternative Names

Bleomycin Hydrolase; BLM Hydrolase; EC 3.4.22.40; BMH; BH;

Function

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.

Biological Process

Homocysteine catabolic process Source: GO_Central
Protein polyubiquitination Source: Reactome
Proteolysis Source: ProtInc
Response to drug Source: Ensembl
Response to toxic substance Source: GO_Central

Cellular Location

Cytoplasm

More Infomation

References

Cheng, S., Khan, M., Luo, L., Wang, L., Liu, S., Ping, J., ... & Hu, Q. (2021). Detection of bleomycin and its hydrolase by the cationic surfactant-doped liquid crystal-based sensing platform. Analytica Chimica Acta, 1150, 338247.

Riise, R., Odqvist, L., Mattsson, J., Monkley, S., Abdillahi, S. M., Tyrchan, C., ... & Yrlid, L. F. (2019). Bleomycin hydrolase regulates the release of chemokines important for inflammation and wound healing by keratinocytes. Scientific reports, 9(1), 1-10.

Wu, H., Li, L., Chen, K. M., Homolka, D., Gos, P., Fleury-Olela, F., ... & Pillai, R. S. (2019). Decapping enzyme NUDT12 partners with BLMH for cytoplasmic surveillance of NAD-capped RNAs. Cell reports, 29(13), 4422-4434.

Crnovcic, I., Gan, F., Yang, D., Dong, L. B., Schultz, P. G., & Shen, B. (2018). Activities of recombinant human bleomycin hydrolase on bleomycins and engineered analogues revealing new opportunities to overcome bleomycin-induced pulmonary toxicity. Bioorganic & medicinal chemistry letters, 28(16), 2670-2674.

Thakkar, D. N., Palugulla, S., Selvarajan, S., & Dubashi, B. (2018). Frequency distribution of BLMH, XPO5 and HFE gene polymorphisms in the South Indian population and their association with Hodgkin Lymphoma. The International journal of biological markers, 33(4), 514-519.

Thakkar, D. (2017). Role of BLMH, XPO5 and HFE gene polymorphisms in Hodgkin lymphoma susceptibility in the South Indian population. Annals of Oncology, 28, x98.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-BLMH Recombinant Antibody (CBYY-1943)

Mouse Anti-BLMH Recombinant Antibody (4A2)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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