Mouse Anti-BMP3 Recombinant Antibody (CBLNB-130) (CBMAB-1216-CN)

Name: Mouse Anti-BMP3 Recombinant Antibody (CBLNB-130)
SKU: CBMAB-1216-CN
Rating: 5 (1 reviews)

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Published Data

CBMAB-1216-CN in IF

Representative immunofluorescence staining images and quantification of the number of PCs90 (Calbindin, green), the integrated intensity of granule cells (GCs), and the integrated intensity of 91 Bergmann glial cells (BGCs, Gdf10, red). ...View More

Wang, F., Wang, Q., Liu, B., Mei, L., Ma, S., Wang, S., ... & Song, X. (2021). The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum. Cell Death & Differentiation, 28(9), 2634-2650.

CBMAB-1216-CN in WB

Protein levels of BMP3, β-catenin, and β-actin were assessed by western blot analysis. ...View More

Kokabu, S., & Rosen, V. (2018). BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling. FEBS Open Bio, 8(2), 168-176.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNB-130

Application

WB, IP, IF, E

Immunogen

Amino acids 363-435 of BMP-3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bone Morphogenetic Protein 3

Introduction

Bone morphogenetic protein 3 (BMP30) is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. BMP30 is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein suppresses osteoblast differentiation, and negatively regulates bone density, by modulating TGF-beta receptor availability to other ligands.

Entrez Gene ID

651

UniProt ID

P12645

Alternative Names

BMP-3A

Function

Growth factor of the TGF-beta superfamily that plays an essential role in developmental process by inducing and patterning early skeletal formation and by negatively regulating bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification (PubMed:11138004, PubMed:15269709).
Initiates signaling cascades by associating with type II receptor ACVR2B to activate SMAD2-dependent and SMAD-independent signaling cascades including TAK1 and JNK pathways (PubMed:31665064).

Biological Process

Cartilage development Source: UniProtKB-KW
Cell-cell signaling Source: ProtInc
Osteoblast differentiation Source: InterPro
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
Skeletal system development Source: ProtInc
SMAD protein signal transduction Source: GO_Central

Cellular Location

Secreted

Mathangi, R., Shyamsundar, V., & Shila, S. (2020). Immunohistochemical evaluation of bone morphogenetic protein 3 in chronic periodontitis influenced by Type2 diabetes mellitus. Drug invention today, 14(1).

TRIASWANTO, F. (2020). Distribusi Bone Morphogenetic Protein 3 (BMP3) pada Regenerat Ekor Tokek (Gekko gecko Linnaeus, 1758) (Doctoral dissertation, Universitas Gadjah Mada).

Fan, L., Fan, J., Liu, Y., Li, T., Xu, H., Yang, Y., ... & Zhao, R. C. (2018). miR-450b promotes osteogenic differentiation in vitro and enhances bone formation in vivo by targeting BMP3. Stem cells and development, 27(9), 600-611.

Agaviezor, B. O., Ajayi, F. O., & Omoyibo, T. W. (2017). Bone Morphogenetic Protein 3 (BMP3) Gene Variation in some Livestock Animals. Nigerian Journal of Biotechnology, 32, 28-32.

Houshmand, M., Abbaszadegan, M. R., & Kerachian, M. A. (2017). Assessment of bone morphogenetic protein 3 methylation in Iranian patients with colorectal cancer. Middle East journal of digestive diseases, 9(3), 158.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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