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Mouse Anti-BRMS1 Recombinant Antibody (2D4-2G11) (CBMAB-A0883-LY)

The product is antibody recognizes BRMS1. The antibody 2D4-2G11 immunoassay techniques such as: WB, ELISA.
See all BRMS1 antibodies
Published Data
Fig.1 Expression of BRMS1 in GC tissues and cell lines, and positive correlation of BRMS1 expression with miR-125a-5p expression level.
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Fig.2 Stable BRMS1-silenced melanoma cell line (sh635) shows a decreased capability of lamellipodia formation compared to WT melanoma cell line.
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Fig.3 293T whole-cell lysates were coimmunoprecipitated with control (IgG), anti-HDAC1, or anti-HDAC3 antibody, followed by immunoblotting for the presence of endogenous BRMS1.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2D4-2G11
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
BRMS1 (AAH09834, 1 a.a. ~ 246 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Breast Cancer Metastasis Suppressor 1
Introduction
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
Entrez Gene ID
25855
UniProt ID
Q9HCU9
Alternative Names
DKFZp564A063
Function
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Biological Process
Apoptotic process Source: UniProtKB-KW
Histone deacetylation Source: GO_Central
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Positive regulation of anoikis Source: UniProtKB
Positive regulation of protein deacetylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Predominantly nuclear.
PTM
Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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