Mouse Anti-BRMS1 Recombinant Antibody (EG413) (CBMAB-EN471-LY)

Name: Mouse Anti-BRMS1 Recombinant Antibody (EG413)
SKU: CBMAB-EN471-LY
Rating: 5 (1 reviews)

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Published Data

CBMAB-EN471-LY in WB

Equal amounts of total cellular proteins from these cells were resolved by SDS-PAGE and processed for immunoblotting with antibodies to c-Abl, MMP3, ErbB2, Snail, BRMS1, Kiss1 and Claudin-1. PG, plakoglobin. ...View More

Aktary, Z., & Pasdar, M. (2013). Plakoglobin represses SATB1 expression and decreases in vitro proliferation, migration and invasion. PloS one, 8(11), e78388.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG413

Application

WB, IP, E

Immunogen

Amino acids 1-247 representing full-length BRMS1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Breast Cancer Metastasis Suppressor 1

Introduction

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	25855
Mouse	107392
Rat	293668

UniProt ID

Human	Q9HCU9
Mouse	Q99N20
Rat	Q5M7T3

Alternative Names

Breast Cancer Metastasis Suppressor 1;

Function

Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.

Biological Process

Apoptotic process Source: UniProtKB-KW
Histone deacetylation Source: GO_Central
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Positive regulation of anoikis Source: UniProtKB
Positive regulation of protein deacetylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Predominantly nuclear.

PTM

Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.

Zimmermann, R. C., & Welch, D. R. (2020). BRMS1: a multifunctional signaling molecule in metastasis. Cancer and Metastasis Reviews, 1-14.

Qiu, R., Shi, H., Wang, S., Leng, S., Liu, R., Zheng, Y., ... & Yang, Y. (2018). BRMS1 coordinates with LSD1 and suppresses breast cancer cell metastasis. American journal of cancer research, 8(10), 2030.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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