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Mouse Anti-BRMS1 Recombinant Antibody (EG413) (CBMAB-EN471-LY)


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Published Data
CBMAB-EN471-LY-1
CBMAB-EN471-LY in WB
Equal amounts of total cellular proteins from these cells were resolved by SDS-PAGE and processed for immunoblotting with antibodies to c-Abl, MMP3, ErbB2, Snail, BRMS1, Kiss1 and Claudin-1. PG, plakoglobin. ...View More
Aktary, Z., & Pasdar, M. (2013). Plakoglobin represses SATB1 expression and decreases in vitro proliferation, migration and invasion. PloS one, 8(11), e78388.

Summary

Host Animal
Mouse
Specificity
Human
Clone
EG413
Antibody Isotype
IgG1, κ
Application
WB, IP, E

Basic Information

Immunogen
Amino acids 1-247 representing full-length BRMS1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Breast Cancer Metastasis Suppressor 1
Introduction
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human25855
Mouse107392
Rat293668
UniProt ID
HumanQ9HCU9
MouseQ99N20
RatQ5M7T3
Alternative Names
Breast Cancer Metastasis Suppressor 1;
Function
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Biological Process
Apoptotic process Source: UniProtKB-KW
Histone deacetylation Source: GO_Central
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Positive regulation of anoikis Source: UniProtKB
Positive regulation of protein deacetylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Predominantly nuclear.
PTM
Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.
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For research use only. Not intended for any clinical use.

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