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Rabbit Anti-BRSK1 Recombinant Antibody (D10F2) (CBMAB-CP0034-LY)

The product is antibody recognizes BRSK1. The antibody D10F2 immunoassay techniques such as: WB,IP.
See all BRSK1 antibodies
Published Data
CBMAB-CP0034-LY-1
CBMAB-CP0034-LY in IF
SadA and SadB can be detected in the CP and axons but not the VZ.
(A) Coronal sections from the brains of E13, E15 or E17 mouse embryos were stained with Hoechst 33342 (blue) and an anti-SadA (green) antibody directed against the N-terminal kinase domain or an anti-Sad B antibody (green) directed against the C-terminus. (B) Cortical neurons from E18 rat embryos were analyzed at 1, 2 and 3 days in vitro (d.i.v., stage 2 to 3) by staining with anti-SadA (A, green), anti-SadB (A, green) and the Tuj1 (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). Early and late stage 3 neurons are shown. Axons are marked by arrowheads. The scale bars are 20 μm (A) and 50 μm, respectively (B). ...View More
Dhumale, P., Menon, S., Chiang, J., & Püschel, A. W. (2018). The loss of the kinases SadA and SadB results in early neuronal apoptosis and a reduced number of progenitors. PLoS One, 13(4), e0196698.
CBMAB-CP0034-LY-2
CBMAB-CP0034-LY in IF
Active SadA and SadB are restricted to the axons of polarized neurons.
(A) Coronal sections from the brains of E17 mouse embryos were stained with Hoechst 33342 (blue) an antibody detecting active SadA and SadB phosphorylated at Thr175 and Thr187, respectively, (P-SadA/B, green) and the Tuj1 (red) antibody. (B) Cortical neurons from E18 rat embryos were analyzed at 1, 2 and 3 days in vitro (d.i.v., stage 2 to 3) by staining Hoechst 33342 (blue) an antibody detecting active SadA and SadB phosphorylated at Thr175 and Thr187, respectively, (P-SadA/B, green) and the Tuj1 (red) antibody. Early and late stage 3 neurons are shown. Axons are marked by arrowheads. The scale bars are 20 μm (A) and 50 μm, respectively (B). ...View More
Dhumale, P., Menon, S., Chiang, J., & Püschel, A. W. (2018). The loss of the kinases SadA and SadB results in early neuronal apoptosis and a reduced number of progenitors. PLoS One, 13(4), e0196698.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D10F2
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly485 of human BRSK1 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BR Serine/Threonine Kinase 1
Introduction
Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at Thr-529 and Ser-579. Also regulates neuron polarization by mediating phosphorylation of WEE1 at Ser-642 in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at Ser-131, leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
Entrez Gene ID
Human84446
Mouse381979
Rat499073
UniProt ID
HumanQ8TDC3
MouseQ5RJI5
RatB2DD29
Alternative Names
BR Serine/Threonine Kinase 1; Brain-Specific Serine/Threonine-Protein Kinase 1; Synapses Of Amphids Defective Homolog 1; Serine/Threonine-Protein Kinase SAD-B; BR Serine/Threonine-Protein Kinase 1; Brain-Selective Kinase 1; SAD1 Homolog; EC 2.7.11.1; HSAD1; Serine/Threonine-Protein Kinase BRSK1;
Function
Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
Biological Process
Associative learning Source: ARUK-UCL
Axonogenesis Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to glucose starvation Source: GO_Central
Centrosome duplication Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G2/M transition of mitotic cell cycle Source: MGI
Intracellular signal transduction Source: GO_Central
Microtubule cytoskeleton organization involved in establishment of planar polarity Source: ARUK-UCL
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neurotransmitter secretion Source: UniProtKB
Peptidyl-serine phosphorylation Source: MGI
Protein phosphorylation Source: UniProtKB
Regulation of axonogenesis Source: ARUK-UCL
Regulation of neuron projection development Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Regulation of synaptic vesicle clustering Source: ARUK-UCL
Response to UV Source: UniProtKB
Synaptic vesicle cycle Source: ARUK-UCL
Cellular Location
Centrosome; Nucleus; Cytoplasm; Synapse; Presynaptic active zone; Synaptic vesicle. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage (By similarity).
PTM
Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C.
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For research use only. Not intended for any clinical use.

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