Rabbit Anti-BRSK1 Recombinant Antibody (D10F2) (CBMAB-CP0034-LY)

Name: Rabbit Anti-BRSK1 Recombinant Antibody (D10F2)
SKU: CBMAB-CP0034-LY
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Published Data

CBMAB-CP0034-LY in IF

SadA and SadB can be detected in the CP and axons but not the VZ.
(A) Coronal sections from the brains of E13, E15 or E17 mouse embryos were stained with Hoechst 33342 (blue) and an anti-SadA (green) antibody directed against the N-terminal kinase domain or an anti-Sad B antibody (green) directed against the C-terminus. (B) Cortical neurons from E18 rat embryos were analyzed at 1, 2 and 3 days in vitro (d.i.v., stage 2 to 3) by staining with anti-SadA (A, green), anti-SadB (A, green) and the Tuj1 (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). Early and late stage 3 neurons are shown. Axons are marked by arrowheads. The scale bars are 20 μm (A) and 50 μm, respectively (B). ...View More

Dhumale, P., Menon, S., Chiang, J., & Püschel, A. W. (2018). The loss of the kinases SadA and SadB results in early neuronal apoptosis and a reduced number of progenitors. PLoS One, 13(4), e0196698.

CBMAB-CP0034-LY in IF

Active SadA and SadB are restricted to the axons of polarized neurons.
(A) Coronal sections from the brains of E17 mouse embryos were stained with Hoechst 33342 (blue) an antibody detecting active SadA and SadB phosphorylated at Thr175 and Thr187, respectively, (P-SadA/B, green) and the Tuj1 (red) antibody. (B) Cortical neurons from E18 rat embryos were analyzed at 1, 2 and 3 days in vitro (d.i.v., stage 2 to 3) by staining Hoechst 33342 (blue) an antibody detecting active SadA and SadB phosphorylated at Thr175 and Thr187, respectively, (P-SadA/B, green) and the Tuj1 (red) antibody. Early and late stage 3 neurons are shown. Axons are marked by arrowheads. The scale bars are 20 μm (A) and 50 μm, respectively (B). ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D10F2

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues surrounding Gly485 of human BRSK1 protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BR Serine/Threonine Kinase 1

Introduction

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at Thr-529 and Ser-579. Also regulates neuron polarization by mediating phosphorylation of WEE1 at Ser-642 in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at Ser-131, leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

Entrez Gene ID

Human	84446
Mouse	381979
Rat	499073

UniProt ID

Human	Q8TDC3
Mouse	Q5RJI5
Rat	B2DD29

Alternative Names

BR Serine/Threonine Kinase 1; Brain-Specific Serine/Threonine-Protein Kinase 1; Synapses Of Amphids Defective Homolog 1; Serine/Threonine-Protein Kinase SAD-B; BR Serine/Threonine-Protein Kinase 1; Brain-Selective Kinase 1; SAD1 Homolog; EC 2.7.11.1; HSAD1; Serine/Threonine-Protein Kinase BRSK1;

Function

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

Biological Process

Associative learning Source: ARUK-UCL
Axonogenesis Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to glucose starvation Source: GO_Central
Centrosome duplication Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G2/M transition of mitotic cell cycle Source: MGI
Intracellular signal transduction Source: GO_Central
Microtubule cytoskeleton organization involved in establishment of planar polarity Source: ARUK-UCL
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neurotransmitter secretion Source: UniProtKB
Peptidyl-serine phosphorylation Source: MGI
Protein phosphorylation Source: UniProtKB
Regulation of axonogenesis Source: ARUK-UCL
Regulation of neuron projection development Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Regulation of synaptic vesicle clustering Source: ARUK-UCL
Response to UV Source: UniProtKB
Synaptic vesicle cycle Source: ARUK-UCL

Cellular Location

Centrosome; Nucleus; Cytoplasm; Synapse; Presynaptic active zone; Synaptic vesicle. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage (By similarity).

PTM

Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C.

Van der Kooi, A. L. L., van Dijk, M., Broer, L., van den Berg, M. H., Laven, J. S., van Leeuwen, F. E., ... & Van Dulmen-den Broeder, E. (2021). Possible modification of BRSK1 on the risk of alkylating chemotherapy-related reduced ovarian function. Human Reproduction, 36(4), 1120-1133.

Su, C. L., Tsai, Y. L., Nfor, O. N., Liu, W. H., Ho, C. C., Lung, C. C., ... & Liaw, Y. P. (2020). Relationship between BRSK1 rs12611091 variant and age at natural menopause based on physical activity. Menopause (New York, NY), 27(4), 459.

Zhou, G., Zhang, X., Wang, W., Zhang, W., Wang, H., & Xin, G. (2019). Both peripheral blood and urinary miR-195-5p, miR-192-3p, miR-328-5p and their target genes PPM1A, RAB1A and BRSK1 may be potential biomarkers for membranous nephropathy. Medical science monitor: international medical journal of experimental and clinical research, 25, 1903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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