Mouse Recombinant BRSK1, Active protein, GST Tag (V2LY-0526-LY7883)

Name: Mouse Recombinant BRSK1, Active protein, GST Tag
SKU: V2LY-0526-LY7883
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Mouse

Tag

GST Tag

Protein Construction

This product is Mouse Recombinant BRSK1, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 118 kDa.

Molecule Mass

118 kDa

Sequence

Amino Acid: Full Length

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

BR Serine/Threonine Kinase 1

Function

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

Biological Process

Associative learning Source: ARUK-UCL
Axonogenesis Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to glucose starvation Source: GO_Central
Centrosome duplication Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G2/M transition of mitotic cell cycle Source: MGI
Intracellular signal transduction Source: GO_Central
Microtubule cytoskeleton organization involved in establishment of planar polarity Source: ARUK-UCL
Mitotic G2 DNA damage checkpoint Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neurotransmitter secretion Source: UniProtKB
Peptidyl-serine phosphorylation Source: MGI
Protein phosphorylation Source: UniProtKB
Regulation of axonogenesis Source: ARUK-UCL
Regulation of neuron projection development Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Regulation of synaptic vesicle clustering Source: ARUK-UCL
Response to UV Source: UniProtKB
Synaptic vesicle cycle Source: ARUK-UCL

Cellular Location

Centrosome; Nucleus; Cytoplasm; Synapse; Presynaptic active zone; Synaptic vesicle. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage (By similarity).

PTM

Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C.

Van der Kooi, A. L. L., van Dijk, M., Broer, L., van den Berg, M. H., Laven, J. S., van Leeuwen, F. E., ... & Van Dulmen-den Broeder, E. (2021). Possible modification of BRSK1 on the risk of alkylating chemotherapy-related reduced ovarian function. Human Reproduction, 36(4), 1120-1133.

Su, C. L., Tsai, Y. L., Nfor, O. N., Liu, W. H., Ho, C. C., Lung, C. C., ... & Liaw, Y. P. (2020). Relationship between BRSK1 rs12611091 variant and age at natural menopause based on physical activity. Menopause (New York, NY), 27(4), 459.

Zhou, G., Zhang, X., Wang, W., Zhang, W., Wang, H., & Xin, G. (2019). Both peripheral blood and urinary miR-195-5p, miR-192-3p, miR-328-5p and their target genes PPM1A, RAB1A and BRSK1 may be potential biomarkers for membranous nephropathy. Medical science monitor: international medical journal of experimental and clinical research, 25, 1903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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