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Mouse Anti-BTC Recombinant Antibody (CBYY-0861) (CBMAB-0864-YY)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBYY-0861
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 1-178 representing full length BTC of human origin.
Host Species
Mouse
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 40-89

Target

Full Name
Betacellulin
Introduction
This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.
Entrez Gene ID
Human685
Mouse12223
Rat64022
UniProt ID
HumanP35070
MouseQ05928
RatQ9JJM4
Alternative Names
Betacellulin; Probetacellulin;
Function
Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Biological Process
Epidermal growth factor receptor signaling pathway Source: GO_Central
ERBB2 signaling pathway Source: Reactome
MAPK cascade Source: Reactome
Membrane organization Source: Reactome
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
Positive regulation of cell differentiation Source: Ensembl
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Positive regulation of fibroblast proliferation Source: Ensembl
Positive regulation of mitotic nuclear division Source: GO_Central
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of urine volume Source: Ensembl
Regulation of cell motility Source: Reactome
Signal transduction Source: Reactome
Cellular Location
Betacellulin: Extracellular space
Probetacellulin: Cell membrane
Topology
Extracellular: 32-118 aa
Helical: 119-139 aa
Cytoplasmic: 140-178 aa
More Infomation

Lee, Y. S., Song, G. J., & Jun, H. S. (2021). Betacellulin-Induced α-Cell Proliferation Is Mediated by ErbB3 and ErbB4, and May Contribute to β-Cell Regeneration. Frontiers in cell and developmental biology, 8, 1788.

Wang, Y., Zhang, F., Zhang, Y., Shan, Q., Liu, W., Zhang, F., ... & Yi, S. (2021). Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Molecular Medicine, 27(1), 1-12.

Hedegger, K., Stumpf, F., Blum, H., Graf, A., Schmid, R. M., Lesina, M., ... & Dahlhoff, M. (2020). The protective effect of betacellulin against acute pancreatitis is ERBB4 dependent. Journal of gastroenterology, 55(3), 317-329.

Hedegger, K., Algül, H., Lesina, M., Blutke, A., Schmid, R. M., Schneider, M. R., & Dahlhoff, M. (2020). Unraveling ERBB network dynamics upon betacellulin signaling in pancreatic ductal adenocarcinoma in mice. Molecular oncology, 14(8), 1653-1669.

Zhang, D., Shen, B., Zhang, Y., Ni, N., Wang, Y., Fan, X., ... & Gu, P. (2018). Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro. Journal of cellular and molecular medicine, 22(1), 330-345.

Olsen, D. A., Kjaer, I. M., & Brandslund, I. (2018). Development of a three-plex single molecule immunoassay enabling measurement of the EGFR ligands amphiregulin, betacellulin and transforming growth factor α simultaneously in human serum samples. Journal of immunological methods, 459, 63-69.

Rush, J. S., Peterson, J. L., & Ceresa, B. P. (2018). Betacellulin (BTC) biases the EGFR to dimerize with ErbB3. Molecular pharmacology, 94(6), 1382-1390.

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For research use only. Not intended for any clinical use.

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