Mouse Anti-BTC Recombinant Antibody (CBYY-0861) (CBMAB-0864-YY)

Name: Mouse Anti-BTC Recombinant Antibody (CBYY-0861)
SKU: CBMAB-0864-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0861

Application

WB, IP, IF, E

Immunogen

Amino acids 1-178 representing full length BTC of human origin.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 40-89

More Infomation

Target

Full Name

Betacellulin

Introduction

This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.

Entrez Gene ID

Human	685
Mouse	12223
Rat	64022

UniProt ID

Human	P35070
Mouse	Q05928
Rat	Q9JJM4

Alternative Names

Betacellulin; Probetacellulin;

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Biological Process

Epidermal growth factor receptor signaling pathway Source: GO_Central
ERBB2 signaling pathway Source: Reactome
MAPK cascade Source: Reactome
Membrane organization Source: Reactome
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
Positive regulation of cell differentiation Source: Ensembl
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Positive regulation of fibroblast proliferation Source: Ensembl
Positive regulation of mitotic nuclear division Source: GO_Central
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of urine volume Source: Ensembl
Regulation of cell motility Source: Reactome
Signal transduction Source: Reactome

Cellular Location

Betacellulin: Extracellular space
Probetacellulin: Cell membrane

Topology

Extracellular: 32-118 aa
Helical: 119-139 aa
Cytoplasmic: 140-178 aa

Lee, Y. S., Song, G. J., & Jun, H. S. (2021). Betacellulin-Induced α-Cell Proliferation Is Mediated by ErbB3 and ErbB4, and May Contribute to β-Cell Regeneration. Frontiers in cell and developmental biology, 8, 1788.

Wang, Y., Zhang, F., Zhang, Y., Shan, Q., Liu, W., Zhang, F., ... & Yi, S. (2021). Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Molecular Medicine, 27(1), 1-12.

Hedegger, K., Stumpf, F., Blum, H., Graf, A., Schmid, R. M., Lesina, M., ... & Dahlhoff, M. (2020). The protective effect of betacellulin against acute pancreatitis is ERBB4 dependent. Journal of gastroenterology, 55(3), 317-329.

Hedegger, K., Algül, H., Lesina, M., Blutke, A., Schmid, R. M., Schneider, M. R., & Dahlhoff, M. (2020). Unraveling ERBB network dynamics upon betacellulin signaling in pancreatic ductal adenocarcinoma in mice. Molecular oncology, 14(8), 1653-1669.

Zhang, D., Shen, B., Zhang, Y., Ni, N., Wang, Y., Fan, X., ... & Gu, P. (2018). Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro. Journal of cellular and molecular medicine, 22(1), 330-345.

Olsen, D. A., Kjaer, I. M., & Brandslund, I. (2018). Development of a three-plex single molecule immunoassay enabling measurement of the EGFR ligands amphiregulin, betacellulin and transforming growth factor α simultaneously in human serum samples. Journal of immunological methods, 459, 63-69.

Rush, J. S., Peterson, J. L., & Ceresa, B. P. (2018). Betacellulin (BTC) biases the EGFR to dimerize with ErbB3. Molecular pharmacology, 94(6), 1382-1390.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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