Mouse Recombinant BTC protein, His & hFc Tag (V2LY-0526-LY7870)

Name: Mouse Recombinant BTC protein, His & hFc Tag
SKU: V2LY-0526-LY7870
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His & hFc Tag

Protein Construction

This product is Mouse Recombinant BTC protein, His & hFc Tag consist of Amino Acid: 1-118 and predicts a molecular mass of 38 kDa.

Molecule Mass

38 kDa

Sequence

Amino Acid: 1-118

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Betacellulin

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Biological Process

Epidermal growth factor receptor signaling pathway Source: GO_Central
ERBB2 signaling pathway Source: Reactome
MAPK cascade Source: Reactome
Membrane organization Source: Reactome
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
Positive regulation of cell differentiation Source: Ensembl
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Positive regulation of fibroblast proliferation Source: Ensembl
Positive regulation of mitotic nuclear division Source: GO_Central
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of urine volume Source: Ensembl
Regulation of cell motility Source: Reactome
Signal transduction Source: Reactome

Cellular Location

Betacellulin: Extracellular space
Probetacellulin: Cell membrane

Topology

Extracellular: 32-118 aa
Helical: 119-139 aa
Cytoplasmic: 140-178 aa

Lee, Y. S., Song, G. J., & Jun, H. S. (2021). Betacellulin-Induced α-Cell Proliferation Is Mediated by ErbB3 and ErbB4, and May Contribute to β-Cell Regeneration. Frontiers in cell and developmental biology, 8, 1788.

Wang, Y., Zhang, F., Zhang, Y., Shan, Q., Liu, W., Zhang, F., ... & Yi, S. (2021). Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Molecular Medicine, 27(1), 1-12.

Hedegger, K., Stumpf, F., Blum, H., Graf, A., Schmid, R. M., Lesina, M., ... & Dahlhoff, M. (2020). The protective effect of betacellulin against acute pancreatitis is ERBB4 dependent. Journal of gastroenterology, 55(3), 317-329.

Hedegger, K., Algül, H., Lesina, M., Blutke, A., Schmid, R. M., Schneider, M. R., & Dahlhoff, M. (2020). Unraveling ERBB network dynamics upon betacellulin signaling in pancreatic ductal adenocarcinoma in mice. Molecular oncology, 14(8), 1653-1669.

Zhang, D., Shen, B., Zhang, Y., Ni, N., Wang, Y., Fan, X., ... & Gu, P. (2018). Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro. Journal of cellular and molecular medicine, 22(1), 330-345.

Olsen, D. A., Kjaer, I. M., & Brandslund, I. (2018). Development of a three-plex single molecule immunoassay enabling measurement of the EGFR ligands amphiregulin, betacellulin and transforming growth factor α simultaneously in human serum samples. Journal of immunological methods, 459, 63-69.

Rush, J. S., Peterson, J. L., & Ceresa, B. P. (2018). Betacellulin (BTC) biases the EGFR to dimerize with ErbB3. Molecular pharmacology, 94(6), 1382-1390.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BTC protein, hFc Tag (CAT#: V2LY-0526-LY2345)

Rhesus Recombinant BTC protein, hFc Tag (CAT#: V2LY-0526-LY9650)

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Human Recombinant BTC protein, hFc Tag (CAT#: V2LY-0526-LY2345)

Rhesus Recombinant BTC protein, hFc Tag (CAT#: V2LY-0526-LY9650)

Mouse Anti-BTC Recombinant Antibody (EG424) (CAT#: CBMAB-EN482-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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