Rat Anti-BTLA Recombinant Antibody (PK18.6) (CBMAB-C8310-LY)

Name: Rat Anti-BTLA Recombinant Antibody (PK18.6)
SKU: CBMAB-C8310-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

PK18.6

Application

In vivo, FC

Immunogen

Mouse BTLA external domain-human IgG fusion protein.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.0

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

B And T Lymphocyte Associated

Introduction

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]

Entrez Gene ID

208154

UniProt ID

Q7TSA3

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050).
May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044).
In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Immune response-regulating cell surface receptor signaling pathway Source: GO_Central
T cell costimulation Source: Reactome

Cellular Location

Cell membrane

Topology

Extracellular: 31-157 aa
Helical: 158-178 aa
Cytoplasmic: 179-289 aa

PTM

Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.

Ning, Z., Liu, K., & Xiong, H. (2021). Roles of BTLA in immunity and immune disorders. Frontiers in Immunology, 12.

Deng, Z., Zheng, Y., Cai, P., & Zheng, Z. (2021). The Role of B and T Lymphocyte Attenuator in Respiratory System Diseases. Frontiers in Immunology, 12, 2141.

Wiedemann, A., Lettau, M., Weißenberg, S. Y., Stefanski, A. L., Schrezenmeier, E. V., Rincon-Arevalo, H., ... & Dörner, T. (2021). BTLA Expression and Function Are Impaired on SLE B Cells. Frontiers in immunology, 12, 1402.

Axelsson, S., Magnuson, A., Lange, A., Alshamari, A., Hörnquist, E. H., & Hultgren, O. (2020). A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex. BMC immunology, 21(1), 1-9.

Zhang, H., Wang, Z., Zhang, J., Zhang, X., Gui, Z., Sun, L., ... & Gu, M. (2020). The synergism of B and T lymphocyte attenuator (BTLA) and cytotoxic T lymphocyte associated antigen-4 (CTLA-4) attenuated acute T-cell mediated rejection and prolonged renal graft survival. Translational Andrology and Urology, 9(5), 1990.

Karabon, L., Partyka, A., Ciszak, L., Pawlak-Adamska, E., Tomkiewicz, A., Bojarska-Junak, A., ... & Kosmaczewska, A. (2020). Abnormal expression of BTLA and CTLA-4 immune checkpoint molecules in chronic lymphocytic leukemia patients. Journal of immunology research, 2020.

Yu, X., Zheng, Y., Mao, R., Su, Z., & Zhang, J. (2019). BTLA/HVEM signaling: milestones in research and role in chronic hepatitis B virus infection. Frontiers in immunology, 10, 617.

Monaghan, S. F., Banerjee, D., Chung, C. S., Lomas-Neira, J., Cygan, K. J., Rhine, C. L., ... & Ayala, A. (2018). Changes in the process of alternative RNA splicing results in soluble B and T lymphocyte attenuator with biological and clinical implications in critical illness. Molecular Medicine, 24(1), 1-7.

Sawaf, M., Fauny, J. D., Felten, R., Sagez, F., Gottenberg, J. E., Dumortier, H., & Monneaux, F. (2018). Defective BTLA functionality is rescued by restoring lipid metabolism in lupus CD4+ T cells. Jci Insight, 3(13).

Lan, X., Li, S., Gao, H., Nanding, A., Quan, L., Yang, C., ... & Xue, Y. (2017). Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. OncoTargets and therapy, 10, 919.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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