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Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836)


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Tested Data
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in ELISA
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in ELISA
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in WB
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in WB
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in WB
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in WB
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in IF
Mouse Anti-BTLA Recombinant Antibody (CBT4810) (V2LY-0625-LY836) in IF

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4810
Antibody Isotype
IgG1
Application
ICC

Basic Information

Immunogen
Purified recombinant fragment of human BTLA (AA: extra 31-157) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ICC1:100-1:500
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
B And T Lymphocyte Associated
Entrez Gene ID
151888
UniProt ID
Q7Z6A9
Function
Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050).
May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044).
In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Immune response-regulating cell surface receptor signaling pathway Source: GO_Central
T cell costimulation Source: Reactome
Cellular Location
Cell membrane
Topology
Extracellular: 31-157 aa
Helical: 158-178 aa
Cytoplasmic: 179-289 aa
PTM
Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.
More Infomation

Ning, Z., Liu, K., & Xiong, H. (2021). Roles of BTLA in immunity and immune disorders. Frontiers in Immunology, 12.

Deng, Z., Zheng, Y., Cai, P., & Zheng, Z. (2021). The Role of B and T Lymphocyte Attenuator in Respiratory System Diseases. Frontiers in Immunology, 12, 2141.

Wiedemann, A., Lettau, M., Weißenberg, S. Y., Stefanski, A. L., Schrezenmeier, E. V., Rincon-Arevalo, H., ... & Dörner, T. (2021). BTLA Expression and Function Are Impaired on SLE B Cells. Frontiers in immunology, 12, 1402.

Axelsson, S., Magnuson, A., Lange, A., Alshamari, A., Hörnquist, E. H., & Hultgren, O. (2020). A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex. BMC immunology, 21(1), 1-9.

Zhang, H., Wang, Z., Zhang, J., Zhang, X., Gui, Z., Sun, L., ... & Gu, M. (2020). The synergism of B and T lymphocyte attenuator (BTLA) and cytotoxic T lymphocyte associated antigen-4 (CTLA-4) attenuated acute T-cell mediated rejection and prolonged renal graft survival. Translational Andrology and Urology, 9(5), 1990.

Karabon, L., Partyka, A., Ciszak, L., Pawlak-Adamska, E., Tomkiewicz, A., Bojarska-Junak, A., ... & Kosmaczewska, A. (2020). Abnormal expression of BTLA and CTLA-4 immune checkpoint molecules in chronic lymphocytic leukemia patients. Journal of immunology research, 2020.

Yu, X., Zheng, Y., Mao, R., Su, Z., & Zhang, J. (2019). BTLA/HVEM signaling: milestones in research and role in chronic hepatitis B virus infection. Frontiers in immunology, 10, 617.

Monaghan, S. F., Banerjee, D., Chung, C. S., Lomas-Neira, J., Cygan, K. J., Rhine, C. L., ... & Ayala, A. (2018). Changes in the process of alternative RNA splicing results in soluble B and T lymphocyte attenuator with biological and clinical implications in critical illness. Molecular Medicine, 24(1), 1-7.

Sawaf, M., Fauny, J. D., Felten, R., Sagez, F., Gottenberg, J. E., Dumortier, H., & Monneaux, F. (2018). Defective BTLA functionality is rescued by restoring lipid metabolism in lupus CD4+ T cells. Jci Insight, 3(13).

Lan, X., Li, S., Gao, H., Nanding, A., Quan, L., Yang, C., ... & Xue, Y. (2017). Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. OncoTargets and therapy, 10, 919.

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For research use only. Not intended for any clinical use.

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