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Mouse Anti-C1GALT1 Recombinant Antibody (1F1) (CBMAB-C3996-LY)


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Published Data
CBMAB-C3996-LY-1
CBMAB-C3996-LY in WB
Cell culture lysates collected after treatment with doxycycline (1 μg/ml) were resolved by SDS-PAGE. Ten micrograms of total lysates were subjected to Western blotting using antibodies against T-synthase and cytochrome c oxidase IV (COX IV), the latter serving as loading control. ...View More
Argüeso, P., Guzman-Aranguez, A., Mantelli, F., Cao, Z., Ricciuto, J., & Panjwani, N. (2009). Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier. Journal of Biological Chemistry, 284(34), 23037-23045.

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F1
Antibody Isotype
IgG2a, κ
Application
E, WB

Basic Information

Immunogen
C1GALT1 (NP_064541, 264 a.a. ~ 363 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
Entrez Gene ID
56913
UniProt ID
Q9NS00
Function
Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Biological Process
Angiogenesis Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Kidney development Source: UniProtKB
O-glycan processing Source: Reactome
O-glycan processing, core 1 Source: GO_Central
Cellular Location
Membrane
Topology
Cytoplasmic: 1-6 aa
Helical: 7-29 aa
Lumenal: 30-363 aa
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For research use only. Not intended for any clinical use.

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