Mouse Anti-C1GALT1 Recombinant Antibody (1F1) (CBMAB-C3996-LY)

Name: Mouse Anti-C1GALT1 Recombinant Antibody (1F1)
SKU: CBMAB-C3996-LY
Rating: 5 (1 reviews)

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Published Data

CBMAB-C3996-LY in WB

Cell culture lysates collected after treatment with doxycycline (1 μg/ml) were resolved by SDS-PAGE. Ten micrograms of total lysates were subjected to Western blotting using antibodies against T-synthase and cytochrome c oxidase IV (COX IV), the latter serving as loading control. ...View More

Argüeso, P., Guzman-Aranguez, A., Mantelli, F., Cao, Z., Ricciuto, J., & Panjwani, N. (2009). Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier. Journal of Biological Chemistry, 284(34), 23037-23045.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F1

Application

E, WB

Immunogen

C1GALT1 (NP_064541, 264 a.a. ~ 363 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1

Entrez Gene ID

56913

UniProt ID

Q9NS00

Function

Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.

Biological Process

Angiogenesis Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Kidney development Source: UniProtKB
O-glycan processing Source: Reactome
O-glycan processing, core 1 Source: GO_Central

Cellular Location

Membrane

Topology

Cytoplasmic: 1-6 aa
Helical: 7-29 aa
Lumenal: 30-363 aa

Nie, R., Cheng, G., Zhang, J., Dong, Y., Wang, C., Liu, J., & Qin, X. (2017). The association of rs1047763 and rs1008898 of C1GALT1 with IgA nephropathy risk: a global meta-analysis. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(3), 95-103.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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