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Mouse Anti-C1GALT1 Recombinant Antibody (CBFYC-0594) (CBMAB-C0648-FY)

This product is mouse antibody that recognizes C1GALT1. The antibody CBFYC-0594 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all C1GALT1 antibodies
Published Data
CBMAB-C0648-FY-1
CBMAB-C0648-FY in WB
The results are presented as a relative fold change in the mRNA expression of the gene in comparison to the gene in the untreated control, where expression was set at 1. ±SD are the mean of triplicate cultures. ** p < 0.01, *** p < 0.001 compared to control. ### p < 0.001 compared to tiliroside monotherapy. The C1GalT1 protein expression was determined by Western blotting. ...View More
Radziejewska, I., Supruniuk, K., Jakimiuk, K., Tomczyk, M., Bielawska, A., & Galicka, A. (2023). Tiliroside Combined with Anti-MUC1 Monoclonal Antibody as Promising Anti-Cancer Strategy in AGS Cancer Cells. International Journal of Molecular Sciences, 24(17), 13036.
CBMAB-C0648-FY-2
CBMAB-C0648-FY in IF
Results of double staining of SLC35A2 (green) and C1GalT1 (red) in wild-type HEK293T cells. Nuclei are shown in blue. Scale bar corresponds to 20 μm. ...View More
Wiertelak, W., Chabowska, K., Szulc, B., Zadorozhna, Y., Olczak, M., & Maszczak-Seneczko, D. (2023). SLC35A2 deficiency reduces protein levels of core 1 β-1, 3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1870(5), 119462.
CBMAB-C0648-FY-3
CBMAB-C0648-FY in WB
Western blotting analysis of C1GalT1 level in wild-type (WT) cells and C1GalT1-deficient (C1GALT1 KO) clone. Ponceau S staining is presented alongside to demonstrate equal loading. Representative data out of three independent experiments are shown. ...View More
Wiertelak, W., Chabowska, K., Szulc, B., Zadorozhna, Y., Olczak, M., & Maszczak-Seneczko, D. (2023). SLC35A2 deficiency reduces protein levels of core 1 β-1, 3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1870(5), 119462.
CBMAB-C0648-FY-4
CBMAB-C0648-FY in IHC
Immunohistological analysis of C1GALT1 protein expression in 26 CCA tissues (Left panel, ×400 magnification); Scale bar, 100 µm and percentage (%) of tumor cases for C1GALT1 expression in CCA tissues (Right panel). ...View More
Khiaowichit, J., Talabnin, C., Dechsukhum, C., Silsirivanit, A., & Talabnin, K. (2022). Down-regulation of C1GALT1 enhances the progression of cholangiocarcinoma through activation of AKT/ERK signaling pathways. Life, 12(2), 174.
CBMAB-C0648-FY-5
CBMAB-C0648-FY in WB
Basal protein expression of C1GALT1 in 4 CCA cell lines (KKU-100, KKU-213A, KKU-213B and KKU-055) and an immortalized human cholangiocyte cell line (MMNK-1). KKU-055 and KK-100 cells were treated with siC1GALT1 and siControl for 48 h. ...View More
Khiaowichit, J., Talabnin, C., Dechsukhum, C., Silsirivanit, A., & Talabnin, K. (2022). Down-regulation of C1GALT1 enhances the progression of cholangiocarcinoma through activation of AKT/ERK signaling pathways. Life, 12(2), 174.
CBMAB-C0648-FY-6
CBMAB-C0648-FY in WB
Expression of C1GalT1 in cell lysates was measured using Western blot analysis. ...View More
Radziejewska, I., Supruniuk, K., Czarnomysy, R., Buzun, K., & Bielawska, A. (2021). Anti-cancer potential of afzelin towards ags gastric cancer cells. Pharmaceuticals, 14(10), 973.
CBMAB-C0648-FY-7
CBMAB-C0648-FY in WB
Effect of RA and anti-MUC1 on ppGAlNAcT2 mRNA and Tn antigen in cell lysates and culture medium in AGS gastric cancer cells. mRNAs were assessed by RT-PCR, proteins by Western blotting. ...View More
Radziejewska, I., Supruniuk, K., & Bielawska, A. (2021). Anti-cancer effect of combined action of anti-MUC1 and rosmarinic acid in AGS gastric cancer cells. European Journal of Pharmacology, 902, 174119.
CBMAB-C0648-FY-8
CBMAB-C0648-FY in WB
The knockout of Cosmc chaperone and the degradation of T-synthase were confirmed by the Western blotting. ...View More
Liu, Z., Liu, J., Dong, X., Hu, X., Jiang, Y., Li, L., ... & Feng, G. (2019). Tn antigen promotes human colorectal cancer metastasis via H-Ras mediated epithelial-mesenchymal transition activation. Journal of Cellular and Molecular Medicine, 23(3), 2083-2092.
CBMAB-C0648-FY-9
CBMAB-C0648-FY in WB
Expression of T-synthase, Cosmc and C3GnT in frozen human colorectal cancer tissues (Tn-positive = 29, Tn-negative = 22) was measured by western blotting. ...View More
Jiang, Y., Liu, Z., Xu, F., Dong, X., Cheng, Y., Hu, Y., ... & An, G. (2018). Aberrant O-glycosylation contributes to tumorigenesis in human colorectal cancer. Journal of cellular and molecular medicine, 22(10), 4875-4885.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0594
Antibody Isotype
IgG2a, κ
Application
E, IF, P, WB, IP

Basic Information

Immunogen
Amino acids 264-364 mapping near the C-terminus of C1GALT1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 264-364

Target

Full Name
core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
Introduction
The protein encoded by this gene generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R.
Entrez Gene ID
56913
UniProt ID
Q9NS00
Alternative Names
C1GALT; T-synthase
Function
Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Biological Process
Angiogenesis Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Kidney development Source: UniProtKB
O-glycan processing Source: Reactome
O-glycan processing, core 1 Source: GO_Central
Cellular Location
Membrane
Topology
Cytoplasmic: 1-6 aa
Helical: 7-29 aa
Lumenal: 30-363 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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