Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY)

Name: Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687)
SKU: CBMAB-C0741-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY) in Immunoblot

Breast cancer cell lines were collected from the monolayer (D0) following 7 days of suspension culture (D7) and analyzed using immunoblotting for markers identified in the top dysregulated cell surface proteins as well as HIF1α. Carbonic Anhydrase IX (CAIX) and ENO2 were significantly upregulated following suspension culture in the cell lines studied. ...View More

Twomey, J. D., & Zhang, B. (2023). Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer. Biomedicines, 11(3), 934.

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY) in WB

CA IX and CA II protein expression levels after the administration of compounds 14, 23, and 66 on HT-29 (A) and compounds 14, 23, and 63 on MCF-7 (B). ...View More

Zengin Kurt, B., Celebi, G., Ozturk Civelek, D., Angeli, A., Akdemir, A., Sonmez, F., & Supuran, C. T. (2023). Tail-approach-based design and synthesis of coumarin-monoterpenes as carbonic anhydrase inhibitors and anticancer agents. ACS omega, 8(6), 5787-5807.

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY) in WB

Protein levels in breast cancer cells assessed by Western-Blot. A representative blot is shown. Results are expressed as mean ± SD of triplicates from three independent experiments. The cells with the highest expression of each pH regulator were used as reference and normalized to 100. *** p < 0.001 compared to reference cells. ...View More

Tavares-Valente, D., Sousa, B., Schmitt, F., Baltazar, F., & Queirós, O. (2021). Disruption of pH dynamics suppresses proliferation and potentiates doxorubicin cytotoxicity in breast cancer cells. Pharmaceutics, 13(2), 242.

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY) in IHC

Immunoexpression of V-ATPase and CAXII in primary invasive breast carcinomas and normal breast epithelium (NBE). Images are in 100× magnification, and the insets are in 200× magnification. The score for immunoreactive extension was score 2:5-50% and score 3:>50% of immunoreactive cells. ...View More

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CBMAB-C0741-FY) in WB

Western blot analysis for CA XII expression and measurements of acidification rate based on intracellular pH (pH i ). Enhanced expression of CA XII proteins in A549 and MDA-MB-231 increased CO2/HCO3 − transporting machinery and, thus, facilitated intracellular acidification rate and provided more acidic intracellular conditions. ...View More

Kim, S. W., Lee, Y. K., Kim, S. H., Park, J. Y., Lee, D. U., Choi, J., ... & Khang, D. (2017). Covalent, non-covalent, encapsulated nanodrug regulate the fate of intra-and extracellular trafficking: impact on cancer and normal cells. Scientific Reports, 7(1), 6454.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0687

Application

E, WB, P, IF, IP

Immunogen

Amino acids 241-354 of CA XII of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Carbonic Anhydrase 12

Introduction

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.CA12 (Carbonic Anhydrase 12) is a Protein Coding gene. Diseases associated with CA12 include Hyperchlorhidrosis, Isolated and Degenerative Disc Disease. Among its related pathways are Nitrogen metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include carbonate dehydratase activity. An important paralog of this gene is CA14.Reversible hydration of carbon dioxide.

Entrez Gene ID

771

UniProt ID

O43570

Alternative Names

Carbonic Anhydrase 12; Carbonic Anhydrase XII; Tumor Antigen HOM-RCC-3.1.3; Carbonate Dehydratase XII; CA-XII; Carbonic Dehydratase

Function

Reversible hydration of carbon dioxide.

Biological Process

Bicarbonate transport Source: Reactome
Chloride ion homeostasis Source: UniProtKB
One-carbon metabolic process Source: GO_Central

Cellular Location

Cell membrane; Membrane

Involvement in disease

Hyperchlorhidrosis, isolated (HYCHL): An autosomal recessive disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.

Topology

Extracellular: 25-301 aa
Helical: 302-322 aa
Cytoplasmic: 323-354 aa

Li, G., Chen, T. W., Nickel, A. C., Muhammad, S., Steiger, H. J., Tzaridis, T., ... & Kahlert, U. D. (2021). Carbonic Anhydrase XII is a Clinically Significant, Molecular Tumor-Subtype Specific Therapeutic Target in Glioma with the Potential to Combat Invasion of Brain Tumor Cells. OncoTargets and therapy, 14, 1707.

Zhao, X., Shen, P., Li, H., Yang, Y., Guo, J., Chen, S., ... & Fang, X. (2020). Carbonic Anhydrase 12 Protects Endplate Cartilage From Degeneration Regulated by IGF-1/PI3K/CREB Signaling Pathway. Frontiers in cell and developmental biology, 8, 1123.

Franke, C. M., Gu, V. W., Grimm, B. G., Cassady, V. C., White, J. R., Weigel, R. J., & Kulak, M. V. (2020). TFAP2C regulates carbonic anhydrase XII in human breast cancer. Oncogene, 39(6), 1290-1301.

Hwang, S., Kang, J. Y., Kim, M. J., Shin, D. M., & Hong, J. H. (2019). Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. Journal of enzyme inhibition and medicinal chemistry, 34(1), 179-188.

Li, Y., Lei, B., Zou, J., Wang, W., Chen, A., Zhang, J., ... & Li, Z. (2019). High expression of carbonic anhydrase 12 (CA12) is associated with good prognosis in breast cancer. Neoplasma, 66(3), 420-426.

Uda, N. R., Stenner, F., Seibert, V., Herzig, P., Markuly, N., Van Dijk, M., ... & Renner, C. (2019). Humanized monoclonal antibody blocking carbonic anhydrase 12 enzymatic activity leads to reduced tumor growth in vitro. Anticancer research, 39(8), 4117-4128.

Hwang, S., Shin, D. M., & Hong, J. H. (2019). Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules, 24(18), 3409.

Avital, D., Hershkovitz, E., & Loewenthal, N. (2018). Exertional rhabdomyolysis in carbonic anhydrase 12 deficiency. Journal of Pediatric Endocrinology and Metabolism, 31(6), 697-699.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CA12 Matched Antibody Pair (163) (CAT#: APMAB-163LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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