Human CA12 ELISA Kit (V2LY-0626-LY3999)

Name: Human CA12 ELISA Kit
SKU: V2LY-0626-LY3999
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.022 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

39.5 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Carbonic Anhydrase 12

Function

Reversible hydration of carbon dioxide.

Biological Process

Bicarbonate transport Source: Reactome
Chloride ion homeostasis Source: UniProtKB
One-carbon metabolic process Source: GO_Central

Cellular Location

Cell membrane; Membrane

Involvement in disease

Hyperchlorhidrosis, isolated (HYCHL): An autosomal recessive disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.

Topology

Extracellular: 25-301 aa
Helical: 302-322 aa
Cytoplasmic: 323-354 aa

Li, G., Chen, T. W., Nickel, A. C., Muhammad, S., Steiger, H. J., Tzaridis, T., ... & Kahlert, U. D. (2021). Carbonic Anhydrase XII is a Clinically Significant, Molecular Tumor-Subtype Specific Therapeutic Target in Glioma with the Potential to Combat Invasion of Brain Tumor Cells. OncoTargets and therapy, 14, 1707.

Zhao, X., Shen, P., Li, H., Yang, Y., Guo, J., Chen, S., ... & Fang, X. (2020). Carbonic Anhydrase 12 Protects Endplate Cartilage From Degeneration Regulated by IGF-1/PI3K/CREB Signaling Pathway. Frontiers in cell and developmental biology, 8, 1123.

Franke, C. M., Gu, V. W., Grimm, B. G., Cassady, V. C., White, J. R., Weigel, R. J., & Kulak, M. V. (2020). TFAP2C regulates carbonic anhydrase XII in human breast cancer. Oncogene, 39(6), 1290-1301.

Hwang, S., Kang, J. Y., Kim, M. J., Shin, D. M., & Hong, J. H. (2019). Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. Journal of enzyme inhibition and medicinal chemistry, 34(1), 179-188.

Li, Y., Lei, B., Zou, J., Wang, W., Chen, A., Zhang, J., ... & Li, Z. (2019). High expression of carbonic anhydrase 12 (CA12) is associated with good prognosis in breast cancer. Neoplasma, 66(3), 420-426.

Uda, N. R., Stenner, F., Seibert, V., Herzig, P., Markuly, N., Van Dijk, M., ... & Renner, C. (2019). Humanized monoclonal antibody blocking carbonic anhydrase 12 enzymatic activity leads to reduced tumor growth in vitro. Anticancer research, 39(8), 4117-4128.

Hwang, S., Shin, D. M., & Hong, J. H. (2019). Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules, 24(18), 3409.

Avital, D., Hershkovitz, E., & Loewenthal, N. (2018). Exertional rhabdomyolysis in carbonic anhydrase 12 deficiency. Journal of Pediatric Endocrinology and Metabolism, 31(6), 697-699.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CA12 Matched Antibody Pair (163) (CAT#: APMAB-163LY)

Human Recombinant CA12 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2563)

Human Recombinant CA12 protein, His Tag (CAT#: V2LY-0526-LY2564)

Human Recombinant CA12 protein, His Tag-1 (CAT#: V2LY-0526-LY2565)

Mouse Recombinant CA12 protein, His Tag (CAT#: V2LY-0526-LY7910)

Antibody Pairs

CA12 Matched Antibody Pair (163) (CAT#: APMAB-163LY)

Related Products

Mouse Recombinant CA12 protein, His Tag (CAT#: V2LY-0526-LY7910)

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Human Recombinant CA12 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2563)

Mouse Anti-CA12 Recombinant Antibody (04) (CAT#: CBMAB-C1738-LY)

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0029) (CAT#: CBMAB-C0036-FY)

Human Recombinant CA12 protein, His Tag (CAT#: V2LY-0526-LY2564)

Mouse Anti-CA12 Recombinant Antibody (2D6E1) (CAT#: CBMAB-C4919-LY)

Human Recombinant CA12 protein, His Tag-1 (CAT#: V2LY-0526-LY2565)

Rabbit Anti-CA12 Recombinant Antibody (D78E4) (CAT#: CBMAB-C5036-LY)

Mouse Anti-CA12 Recombinant Antibody (CBFYC-0687) (CAT#: CBMAB-C0741-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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