Mouse Anti-CACYBP Recombinant Antibody (CBYY-C0863) (CBMAB-C2299-YY)

Name: Mouse Anti-CACYBP Recombinant Antibody (CBYY-C0863)
SKU: CBMAB-C2299-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C0863

Application

WB, IP, IF, E, P

Immunogen

Amino acids 1-228 representing full length CacyBP of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcyclin Binding Protein

Entrez Gene ID

27101

UniProt ID

Q9HB71

Alternative Names

Calcium Binding And Coiled-Coil Domain 2; Antigen Nuclear Dot 52 KDa Protein; Nuclear Domain 10 Protein NDP52; Nuclear Domain 10 Protein 52; Nuclear Dot Protein 52; NDP52; Calcium-Binding And Coiled-Coil Domain-Containing Protein 2;

Function

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).

Biological Process

Aging Source: Ensembl
Cardiac muscle cell differentiation Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to leukemia inhibitory factor Source: Ensembl
Heart development Source: GO_Central
Negative regulation of cell death Source: Ensembl
Positive regulation of DNA replication Source: Ensembl
Response to growth hormone Source: Ensembl

Cellular Location

Nucleus; Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.

PTM

Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.

Zheng, H., & Chen, C. (2021). Downregulation of CacyBP by CRISPR/dCas9-KRAB Prevents Bladder Cancer Progression. Frontiers in Molecular Biosciences, 8, 557.

Xu, Y. J., Hu, Y. M., Qin, C., Wang, F., Cao, W., Yu, Y. W., ... & He, J. (2021). CacyBP promotes the proliferation and invasion of non-small cell lung cancer. Zhonghua Zhong liu za zhi [Chinese Journal of Oncology], 43(9), 924-931.

Lian, Y. F., Huang, Y. L., Zhang, Y. J., Chen, D. M., Wang, J. L., Wei, H., ... & Huang, Y. H. (2019). CACYBP enhances cytoplasmic retention of P27Kip1 to promote hepatocellular carcinoma progression in the absence of RNF41 mediated degradation. Theranostics, 9(26), 8392.

Kasacka, I., Piotrowska, Ż., Weresa, J., & Filipek, A. (2018). Comparative evaluation of CacyBP/SIP protein, β-catenin, and immunoproteasome subunit LMP7 in the heart of rats with hypertension of different etiology. Experimental Biology and Medicine, 243(15-16), 1199-1206.

Feng, S., Zhou, Q., Yang, B., Li, Q., Liu, A., Zhao, Y., ... & Zhai, H. (2018). The effect of S100A6 on nuclear translocation of CacyBP/SIP in colon cancer cells. Plos one, 13(3), e0192208.

Yan, S., Li, A., & Liu, Y. (2018). CacyBP/SIP inhibits the migration and invasion behaviors of glioblastoma cells through activating Siah1 mediated ubiquitination and degradation of cytoplasmic p27. Cell biology international, 42(2), 216-226.

Topolska-Woś, A. M., Rosińska, S., & Filipek, A. (2017). MAP kinase p38 is a novel target of CacyBP/SIP phosphatase. Amino Acids, 49(6), 1069-1076.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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