Mouse Anti-CALR3 Recombinant Antibody (1D1) (CBMAB-C3792-LY)

Name: Mouse Anti-CALR3 Recombinant Antibody (1D1)
SKU: CBMAB-C3792-LY
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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D1

Application

Immunogen

Full length human recombinant protein of human CALR3 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:4,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Calreticulin 3

Introduction

The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011]

Entrez Gene ID

125972

UniProt ID

Q96L12

Alternative Names

Calreticulin 3; Calsperin; Cancer/Testis Antigen 93; Calreticulin-2; CRT2; Testis Secretory Sperm-Binding Protein Li 226n; Calreticulin-3; CMH19; CT93;

Function

During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity).
CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.

Biological Process

Cell differentiation Source: UniProtKB-KW
Endoplasmic reticulum unfolded protein response Source: GO_Central
Protein folding Source: GO_Central
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum lumen

Verhagen, J. M., Veldman, J. H., van der Zwaag, P. A., Jan, H., Brosens, E., Christiaans, I., ... & van de Laar, I. M. (2018). Lack of evidence for a causal role of CALR3 in monogenic cardiomyopathy. European Journal of Human Genetics, 26(11), 1603-1610.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CALR3 Matched Antibody Pair (174) (CAT#: APMAB-174LY)

CALR3 Matched Antibody Pair (175) (CAT#: APMAB-175LY)

Related Products

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CALR3 Matched Antibody Pair (175) (CAT#: APMAB-175LY)

Mouse Anti-CALR3 Recombinant Antibody (CBXC-1097) (CAT#: CBMAB-C1363-CQ)

CALR3 Matched Antibody Pair (174) (CAT#: APMAB-174LY)

Mouse Anti-CALR3 Recombinant Antibody (CBFYC-0764) (CAT#: CBMAB-C0819-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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