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Mouse Anti-CALR3 Recombinant Antibody (CBFYC-0764) (CBMAB-C0819-FY)


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Published Data
CBMAB-C0819-FY-1
Mouse Anti-CALR3 Recombinant Antibody (CBFYC-0764) (CBMAB-C0819-FY) in WB
Western blot analysis probed with anti-GPAT and DGAT. ...View More
Guillory, B., Jawanmardi, N., Iakova, P., Anderson, B., Zang, P., Timchenko, N. A., & Garcia, J. M. (2018). Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPα-p300/DGAT1 pathway. Aging cell, 17(1), e12688.
CBMAB-C0819-FY-2
Mouse Anti-CALR3 Recombinant Antibody (CBFYC-0764) (CBMAB-C0819-FY) in WB
Nuclear extracts from old WT and GKO mice were separated by size exclusion chromatography (SEC), and location of C/EBPα and p300 proteins was determined using Western blotting assay. The membranes were reprobed with antibodies to calreticulin. Positions of markers of SEC are shown on the top. Positions of 42kD and 30-kD isoforms of C/EBPα are shown by arrows. ...View More
Guillory, B., Jawanmardi, N., Iakova, P., Anderson, B., Zang, P., Timchenko, N. A., & Garcia, J. M. (2018). Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPα-p300/DGAT1 pathway. Aging cell, 17(1), e12688.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0764
Antibody Isotype
IgG2b, κ
Application
ELISA, WB, IP

Basic Information

Immunogen
Recombinant CALR3 protein of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calreticulin 3
Introduction
The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy.
Entrez Gene ID
Human125972
Mouse73316
Rat364529
Dog476680
UniProt ID
HumanQ96L12
MouseQ9D9Q6
RatQ6AYG8
DogE2RGY6
Alternative Names
Calreticulin 3; Calsperin; Cancer/Testis Antigen 93; Calreticulin-2; CRT2; Testis Secretory Sperm-Binding Protein Li 226n; Calreticulin-3; CMH19; CT93
Function
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity).
CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
Biological Process
Cell differentiation Source: UniProtKB-KW
Endoplasmic reticulum unfolded protein response Source: GO_Central
Protein folding Source: GO_Central
Spermatogenesis Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum lumen
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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