Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793) (CBMAB-C0848-FY)

Name: Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793)
SKU: CBMAB-C0848-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793) (CBMAB-C0848-FY) in WB

Change in CAPN1 and CAPN2 protein levels in NMDA-treated neurons. Quantitative results of relative CAPN1 or CAPN2 level normalized to β-Tubulin were shown at right (CAPN1: p = 0.0037, N = 7; CAPN2: p < 0.0001, N = 5) ...View More

Wang, L. H., Lin, C. Y., Lin, Y. M., Buée, L., Sergeant, N., Blum, D., ... & Wang, G. S. (2022). Calpain-2 mediates MBNL2 degradation and a developmental RNA processing program in neurodegeneration. Journal of Neuroscience, 42(25), 5102-5114.

Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793) (CBMAB-C0848-FY) in IF

Subcellular distribution of endogenous CAPN2 in mature hippocampal neurons by biochemical fractionation (left) and immunofluorescence staining (right). ...View More

Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793) (CBMAB-C0848-FY) in IF

Cultured hippocampal neurons were cotransfected with an mRFP expression plasmid and shCtrl, or shCAPN2 and stained for Calpain 2. Scale bar, 10 µm. The relative mean fluorescence intensity is plotted. ≥ 8 images per condition; ****P < 0.0001; Student's t-test. ...View More

Liu, G. T., Kochlamazashvili, G., Puchkov, D., Müller, R., Schultz, C., Mackintosh, A. I., ... & Soykan, T. (2022). Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission. The EMBO Journal, 41(9), e109352.

Mouse Anti-CAPN2 Recombinant Antibody (CBFYC-0793) (CBMAB-C0848-FY) in IHC

IHC staining of stress fiber/focal adhesion-related proteins (n = 3 biological replicates). ...View More

Chen, L., Luo, S., Dupre, A., Vasoya, R. P., Parthasarathy, A., Aita, R., ... & Verzi, M. P. (2021). The nuclear receptor HNF4 drives a brush border gene program conserved across murine intestine, kidney, and embryonic yolk sac. Nature communications, 12(1), 2886.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0793

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 2-27 at the N-terminus of Calpain 2 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calpain 2

Introduction

CAPN2 (Calpain 2) is a Protein Coding gene. Diseases associated with CAPN2 include Alzheimer Disease. Among its related pathways are Integrin Pathway and Necroptosis. Gene Ontology (GO) annotations related to this gene include calcium ion binding and peptidase activity. An important paralog of this gene is CAPN1.

Entrez Gene ID

Human	824
Cattle	281662
Rat	29154
Pig	397393
Rabbit	100009092

UniProt ID

Human	P17655
Cattle	Q27971
Rat	Q07009
Pig	P43367
Rabbit	P06814

Alternative Names

Calpain 2; Calcium-Activated Neutral Proteinase 2; Calpain 2, (M/II) Large Subunit; Millimolar-Calpain; Calpain M-Type; EC 3.4.22.53; M-Calpain; CANP 2; CANPL2; Calpain 2, Large [Catalytic] Subunit

Function

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).

Biological Process

Behavioral response to pain Source: Ensembl
Blastocyst development Source: Ensembl
Cellular response to amino acid stimulus Source: UniProtKB
Cellular response to interferon-beta Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Extracellular matrix disassembly Source: Reactome
Female pregnancy Source: Ensembl
Myoblast fusion Source: Ensembl
Positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
Positive regulation of myoblast fusion Source: Ensembl
Positive regulation of neuron death Source: Ensembl
Positive regulation of phosphatidylcholine biosynthetic process Source: Ensembl
Protein autoprocessing Source: Ensembl
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of cytoskeleton organization Source: BHF-UCL
Regulation of interleukin-6 production Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to hypoxia Source: Ensembl

Cellular Location

Cytoplasm; Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Kwon, S. H., Wi, T., Park, Y. I., Kim, M. W., Lee, G., Higaki, T., ... & Lee, R. (2020). Noninvasive Early Detection of Calpain 2-Enriched Non-Small Cell Lung Cancer Using a Human Serum Albumin-Bounded Calpain 2 Nanosensor. Bioconjugate chemistry, 31(3), 803-812.

Shinkai-Ouchi, F., Shindo, M., Doi, N., Hata, S., & Ono, Y. (2020). Calpain-2 participates in the process of calpain-1 inactivation. Bioscience reports, 40(11), BSR20200552.

Cicognola, C., Satir, T. M., Brinkmalm, G., Matečko-Burmann, I., Agholme, L., Bergström, P., ... & Höglund, K. (2020). Tauopathy-associated tau fragment ending at amino acid 224 is generated by Calpain-2 cleavage. Journal of Alzheimer's Disease, 74(4), 1143-1156.

Siuda, D., Randriamboavonjy, V., & Fleming, I. (2019). Regulation of calpain 2 expression by miR-223 and miR-145. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(10), 194438.

Liu, Z. F., Ji, J. J., Zheng, D., Su, L., & Peng, T. (2019). Calpain‐2 protects against heat stress‐induced cardiomyocyte apoptosis and heart dysfunction by blocking p38 mitogen‐activated protein kinase activation. Journal of cellular physiology, 234(7), 10761-10770.

Zheng, D., Su, Z., Zhang, Y., Ni, R., Fan, G. C., Robbins, J., ... & Peng, T. (2019). Calpain-2 promotes MKP-1 expression protecting cardiomyocytes in both in vitro and in vivo mouse models of doxorubicin-induced cardiotoxicity. Archives of toxicology, 93(4), 1051-1065.

Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., & Zhou, J. (2018). Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncology reports, 40(4), 1937-1946.

Liu, B., Zhou, Y., Lu, D., Liu, Y., Zhang, S. Q., Xu, Y., ... & Gu, X. (2017). Comparison of the protein expression of calpain‑1, calpain‑2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncology letters, 14(3), 3705-3710.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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