Rat Capn2 ELISA Kit (V2LY-0626-LY1569)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.021 ng/mL
Detection Range
0.05-30 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
79.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Calpain 2
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Biological Process
Behavioral response to pain Source: Ensembl
Blastocyst development Source: Ensembl
Cellular response to amino acid stimulus Source: UniProtKB
Cellular response to interferon-beta Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Extracellular matrix disassembly Source: Reactome
Female pregnancy Source: Ensembl
Myoblast fusion Source: Ensembl
Positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
Positive regulation of myoblast fusion Source: Ensembl
Positive regulation of neuron death Source: Ensembl
Positive regulation of phosphatidylcholine biosynthetic process Source: Ensembl
Protein autoprocessing Source: Ensembl
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of cytoskeleton organization Source: BHF-UCL
Regulation of interleukin-6 production Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to hypoxia Source: Ensembl
Cellular Location
Cytoplasm; Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Kwon, S. H., Wi, T., Park, Y. I., Kim, M. W., Lee, G., Higaki, T., ... & Lee, R. (2020). Noninvasive Early Detection of Calpain 2-Enriched Non-Small Cell Lung Cancer Using a Human Serum Albumin-Bounded Calpain 2 Nanosensor. Bioconjugate chemistry, 31(3), 803-812.

Shinkai-Ouchi, F., Shindo, M., Doi, N., Hata, S., & Ono, Y. (2020). Calpain-2 participates in the process of calpain-1 inactivation. Bioscience reports, 40(11), BSR20200552.

Cicognola, C., Satir, T. M., Brinkmalm, G., Matečko-Burmann, I., Agholme, L., Bergström, P., ... & Höglund, K. (2020). Tauopathy-associated tau fragment ending at amino acid 224 is generated by Calpain-2 cleavage. Journal of Alzheimer's Disease, 74(4), 1143-1156.

Siuda, D., Randriamboavonjy, V., & Fleming, I. (2019). Regulation of calpain 2 expression by miR-223 and miR-145. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(10), 194438.

Liu, Z. F., Ji, J. J., Zheng, D., Su, L., & Peng, T. (2019). Calpain‐2 protects against heat stress‐induced cardiomyocyte apoptosis and heart dysfunction by blocking p38 mitogen‐activated protein kinase activation. Journal of cellular physiology, 234(7), 10761-10770.

Zheng, D., Su, Z., Zhang, Y., Ni, R., Fan, G. C., Robbins, J., ... & Peng, T. (2019). Calpain-2 promotes MKP-1 expression protecting cardiomyocytes in both in vitro and in vivo mouse models of doxorubicin-induced cardiotoxicity. Archives of toxicology, 93(4), 1051-1065.

Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., & Zhou, J. (2018). Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncology reports, 40(4), 1937-1946.

Liu, B., Zhou, Y., Lu, D., Liu, Y., Zhang, S. Q., Xu, Y., ... & Gu, X. (2017). Comparison of the protein expression of calpain‑1, calpain‑2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncology letters, 14(3), 3705-3710.

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For research use only. Not intended for any clinical use.

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