Mouse Anti-CASP9 Recombinant Antibody (LAP6 96-2-22) (CBMAB-0169CQ)

Name: Mouse Anti-CASP9 Recombinant Antibody (LAP6 96-2-22)
SKU: CBMAB-0169CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CASP9 Recombinant Antibody (LAP6 96-2-22) (CBMAB-0169CQ) in WB

Representative western blot results showing protein levels of cleaved caspase 9, cleaved caspase 3, and cleaved PARP in HepG2 and Huh7 cells treated with HHT at the indicated concentration. ...View More

Wang, H., Wang, R., Huang, D., Li, S., Gao, B., Kang, Z., ... & Yan, J. (2021). Homoharringtonine exerts anti-tumor effects in hepatocellular carcinoma through activation of the hippo pathway. Frontiers in pharmacology, 12, 592071.

Mouse Anti-CASP9 Recombinant Antibody (LAP6 96-2-22) (CBMAB-0169CQ) in WB

Western blots showing stimulation of KIF1Bβ expression but no changes in ALIX, BclxL, Mcl1, Bcl2, EglN3, JNK, phosphorylated JNK levels and no cleavage of caspase 3 (Casp 3), caspase 9 (Casp 9) or PARP in HEK-293 and SH-SY5Y cells following 48 h transfection with NF-YAx but not NF-YAl expression vector (30 μg). ...View More

Cappabianca, L., Farina, A. R., Di Marcotullio, L., Infante, P., De Simone, D., Sebastiano, M., & Mackay, A. R. (2019). Discovery, characterization and potential roles of a novel NF-YA x splice variant in human neuroblastoma. Journal of Experimental & Clinical Cancer Research, 38, 1-25.

Mouse Anti-CASP9 Recombinant Antibody (LAP6 96-2-22) (CBMAB-0169CQ) in WB

Cells were harvested and cell lysates were examined for the expression of full-length PARP (fl-PARP), cleaved PARP (cl-PARP), cleaved Caspase-9 (cl-Casp9) and full-length caspase-3 (Fl-Casp3) and cleaved Caspase-3 (cl-Casp3). Tubulin was used as a loading control. ...View More

Zhang, H. P., Li, G. Q., Guo, W. Z., Chen, G. H., Tang, H. W., Yan, B., ... & Zhang, S. J. (2017). Oridonin synergistically enhances JQ1-triggered apoptosis in hepatocellular cancer cells through mitochondrial pathway. Oncotarget, 8(63), 106833.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

LAP6 96-2-22

Application

IP, WB, ELISA

Immunogen

Recombinant human Caspase 9 pro-domain protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Caspase 9

Introduction

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family.Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules.

Entrez Gene ID

842

UniProt ID

P55211

Alternative Names

Caspase 9, Apoptosis-Related Cysteine Peptidase; ICE-Like Apoptotic Protease 6; ICE-LAP6; APAF-3; MCH6; Caspase 9, Apoptosis-Related Cysteine Protease; Protein Phosphatase 1, Regulatory Subunit 56; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; Protein Phosphatase 1; Regulatory Subunit 56; EC 3.4.22.62; PPP1R56; CASP-9; APAF3

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: Reactome
Apoptotic process Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to UV Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Platelet formation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of response to DNA damage stimulus Source: UniProtKB
Signal transduction in response to DNA damage Source: UniProtKB

Cellular Location

Apoptosome; Cytosol; Cytoplasm; Protein-containing complex

PTM

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

Han, J., Goldstein, L. A., Hou, W., Watkins, S. C., & Rabinowich, H. (2021). Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy, 17(6), 1393-1409.

Doke, T., Huang, S., Qiu, C., Sheng, X., Seasock, M., Liu, H., ... & Susztak, K. (2021). Genome-wide association studies identify the role of caspase-9 in kidney disease. Science advances, 7(45), eabi8051.

Liao, G., Wang, R., & Tang, D. D. (2021). Plk1 Regulates Caspase-9 Phosphorylation at Ser-196 and Apoptosis of Human Airway Smooth Muscle Cells. American journal of respiratory cell and molecular biology, (ja).

Avrutsky, M. I., & Troy, C. M. (2021). Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease. Frontiers in Pharmacology, 12, 1728.

An, H. K., Chung, K. M., Park, H., Hong, J., Gim, J. E., Choi, H., ... & Yu, S. W. (2020). CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis. Autophagy, 16(9), 1598-1617.

Han, C., Liu, Z., Zhang, Y., Shen, A., Dong, C., Zhang, A., ... & Fu, Y. X. (2020). Tumor cells suppress radiation-induced immunity by hijacking caspase 9 signaling. Nature immunology, 21(5), 546-554.

Zhang, L., Zhang, X., Wang, X., He, M., & Qiao, S. (2019). MicroRNA-224 promotes tumorigenesis through downregulation of caspase-9 in triple-negative breast cancer. Disease markers, 2019.

Ercan, S., Arinc, S., Yilmaz, S. G., Altunok, C., Yaman, F., & Isbir, T. (2019). Investigation of caspase 9 gene polymorphism in patients with non-small cell lung cancer. Anticancer research, 39(5), 2437-2441.

Iwai, N., Yasui, K., Tomie, A., Gen, Y., Terasaki, K., Kitaichi, T., ... & Itoh, Y. (2018). Oncogenic miR-96-5p inhibits apoptosis by targeting the caspase-9 gene in hepatocellular carcinoma. International journal of oncology, 53(1), 237-245.

Lu, J., Peng, J., Xiang, M., He, L., Wang, D., Xiong, G., & Li, S. (2018). Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway. Bioscience reports, 38(5), BSR20180071.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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