Mouse Casp9 ELISA Kit (V2LY-0626-LY1027)

Name: Mouse Casp9 ELISA Kit
SKU: V2LY-0626-LY1027
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0033 ng/mL

Detection Range

0.007-1.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

50.0 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Caspase 9

Alternative Names

CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: Reactome
Apoptotic process Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to UV Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Platelet formation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of response to DNA damage stimulus Source: UniProtKB
Signal transduction in response to DNA damage Source: UniProtKB

Cellular Location

Apoptosome; Cytosol; Cytoplasm; Protein-containing complex

PTM

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

Han, J., Goldstein, L. A., Hou, W., Watkins, S. C., & Rabinowich, H. (2021). Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy, 17(6), 1393-1409.

Doke, T., Huang, S., Qiu, C., Sheng, X., Seasock, M., Liu, H., ... & Susztak, K. (2021). Genome-wide association studies identify the role of caspase-9 in kidney disease. Science advances, 7(45), eabi8051.

Liao, G., Wang, R., & Tang, D. D. (2021). Plk1 Regulates Caspase-9 Phosphorylation at Ser-196 and Apoptosis of Human Airway Smooth Muscle Cells. American journal of respiratory cell and molecular biology, (ja).

Avrutsky, M. I., & Troy, C. M. (2021). Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease. Frontiers in Pharmacology, 12, 1728.

An, H. K., Chung, K. M., Park, H., Hong, J., Gim, J. E., Choi, H., ... & Yu, S. W. (2020). CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis. Autophagy, 16(9), 1598-1617.

Han, C., Liu, Z., Zhang, Y., Shen, A., Dong, C., Zhang, A., ... & Fu, Y. X. (2020). Tumor cells suppress radiation-induced immunity by hijacking caspase 9 signaling. Nature immunology, 21(5), 546-554.

Zhang, L., Zhang, X., Wang, X., He, M., & Qiao, S. (2019). MicroRNA-224 promotes tumorigenesis through downregulation of caspase-9 in triple-negative breast cancer. Disease markers, 2019.

Ercan, S., Arinc, S., Yilmaz, S. G., Altunok, C., Yaman, F., & Isbir, T. (2019). Investigation of caspase 9 gene polymorphism in patients with non-small cell lung cancer. Anticancer research, 39(5), 2437-2441.

Iwai, N., Yasui, K., Tomie, A., Gen, Y., Terasaki, K., Kitaichi, T., ... & Itoh, Y. (2018). Oncogenic miR-96-5p inhibits apoptosis by targeting the caspase-9 gene in hepatocellular carcinoma. International journal of oncology, 53(1), 237-245.

Lu, J., Peng, J., Xiang, M., He, L., Wang, D., Xiong, G., & Li, S. (2018). Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway. Bioscience reports, 38(5), BSR20180071.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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